Expression of p75(NGFR) TrkA, and TrkB mRNA in rat C6 glioma and type I astrocyte cultures

L. A. Hutton, J. DeVellis, J. R. Perez-Polo

    Research output: Contribution to journalArticle

    94 Citations (Scopus)

    Abstract

    Using a quantitative reverse transcription-polymerase chain reaction (RT- PCR) we have investigated the expression of the neurotrophin receptors p75(NGFR), trkA, and trkB mRNAs in cultures of rat pup type I astrocytes and in the C6 rat glioma cell line. All three neurotrophin receptor mRNAs are expressed in both C6 cells and in type I astrocytic cultures. p75(NGFR) mRNA levels are increased by either cycloheximide or nerve growth factor (NGF) treatment of C6 cells as measured using RT-PCR. Type I astrocyte cultures also expressed p75(NGFR) mRNA and NGF treatment increased p75(NGFR) mRNA levels in these cultures. TrkB mRNA levels were increased by cycloheximide treatment of type I astrocyte cultures but not by NGF treatment. Using RT- PCR, trkA mRNA was detected in astrocytic cultures as well as in the rat C6 and PC-12 cell lines. We conclude that cultures of type I astrocytes express active NGF receptors and that glia can elicit a response to NGF as seen by an increase in p75(NGFR) mRNA levels following exposure to NGF.

    Original languageEnglish (US)
    Pages (from-to)375-383
    Number of pages9
    JournalJournal of Neuroscience Research
    Volume32
    Issue number3
    DOIs
    StatePublished - 1992

    Fingerprint

    Glioma
    Astrocytes
    Nerve Growth Factor
    Messenger RNA
    Nerve Growth Factor Receptors
    Cycloheximide
    Polymerase Chain Reaction
    Reverse Transcription
    Nerve Growth Factor Receptor
    Cell Line
    Neuroglia

    Keywords

    • astrocyte
    • glia
    • nerve growth factor
    • nerve growth factor receptor

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Expression of p75(NGFR) TrkA, and TrkB mRNA in rat C6 glioma and type I astrocyte cultures. / Hutton, L. A.; DeVellis, J.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 32, No. 3, 1992, p. 375-383.

    Research output: Contribution to journalArticle

    Hutton, L. A. ; DeVellis, J. ; Perez-Polo, J. R. / Expression of p75(NGFR) TrkA, and TrkB mRNA in rat C6 glioma and type I astrocyte cultures. In: Journal of Neuroscience Research. 1992 ; Vol. 32, No. 3. pp. 375-383.
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