Expression of phytase gene in Bombyx mori

Wen Bing Wang, Bin Yao, Qing Li Xiao, Ping Ji, Sheng Peng Wang, Jia Lu He, Xiang Fu Wu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.

Original languageEnglish (US)
Pages (from-to)112-115
Number of pages4
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume19
Issue number1
StatePublished - Jan 2003
Externally publishedYes

Fingerprint

6-Phytase
Bombyx
Gene Expression
Pupa
Hemolymph
Baculoviridae
Larva
Genes
Viruses
Recombinant DNA
Aspergillus niger
Southern Blotting
DNA

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Wang, W. B., Yao, B., Xiao, Q. L., Ji, P., Wang, S. P., He, J. L., & Wu, X. F. (2003). Expression of phytase gene in Bombyx mori. Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, 19(1), 112-115.

Expression of phytase gene in Bombyx mori. / Wang, Wen Bing; Yao, Bin; Xiao, Qing Li; Ji, Ping; Wang, Sheng Peng; He, Jia Lu; Wu, Xiang Fu.

In: Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, Vol. 19, No. 1, 01.2003, p. 112-115.

Research output: Contribution to journalArticle

Wang, WB, Yao, B, Xiao, QL, Ji, P, Wang, SP, He, JL & Wu, XF 2003, 'Expression of phytase gene in Bombyx mori', Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, vol. 19, no. 1, pp. 112-115.
Wang WB, Yao B, Xiao QL, Ji P, Wang SP, He JL et al. Expression of phytase gene in Bombyx mori. Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology. 2003 Jan;19(1):112-115.
Wang, Wen Bing ; Yao, Bin ; Xiao, Qing Li ; Ji, Ping ; Wang, Sheng Peng ; He, Jia Lu ; Wu, Xiang Fu. / Expression of phytase gene in Bombyx mori. In: Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology. 2003 ; Vol. 19, No. 1. pp. 112-115.
@article{c3482bbc27644917a1454058fa3ddcb7,
title = "Expression of phytase gene in Bombyx mori",
abstract = "Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.",
author = "Wang, {Wen Bing} and Bin Yao and Xiao, {Qing Li} and Ping Ji and Wang, {Sheng Peng} and He, {Jia Lu} and Wu, {Xiang Fu}",
year = "2003",
month = "1",
language = "English (US)",
volume = "19",
pages = "112--115",
journal = "Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology",
issn = "1000-3061",
publisher = "Chinese Academy of Sciences",
number = "1",

}

TY - JOUR

T1 - Expression of phytase gene in Bombyx mori

AU - Wang, Wen Bing

AU - Yao, Bin

AU - Xiao, Qing Li

AU - Ji, Ping

AU - Wang, Sheng Peng

AU - He, Jia Lu

AU - Wu, Xiang Fu

PY - 2003/1

Y1 - 2003/1

N2 - Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.

AB - Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.

UR - http://www.scopus.com/inward/record.url?scp=33745655288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745655288&partnerID=8YFLogxK

M3 - Article

C2 - 15969047

AN - SCOPUS:33745655288

VL - 19

SP - 112

EP - 115

JO - Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

JF - Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

SN - 1000-3061

IS - 1

ER -