Expression of phytase gene in Bombyx mori

Wen Bing Wang, Bin Yao, Qing Li Xiao, Ping Ji, Sheng Peng Wang, Jia Lu He, Xiang Fu Wu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.

Original languageEnglish (US)
Pages (from-to)112-115
Number of pages4
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume19
Issue number1
StatePublished - Jan 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Wang, W. B., Yao, B., Xiao, Q. L., Ji, P., Wang, S. P., He, J. L., & Wu, X. F. (2003). Expression of phytase gene in Bombyx mori. Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, 19(1), 112-115.