Expression of RNA CCUG repeats dysregulates translation and degradation of proteins in myotonic dystrophy 2 patients

Elizabeth Salisbury, Benedikt Schoser, Christiane Schneider-Gold, Guo Li Wang, Claudia Huichalaf, Bingwen Jin, Mario Sirito, Partha Sarkar, Ralf Krahe, Nikolai A. Timchenko, Lubov T. Timchenko

Research output: Contribution to journalArticle

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Abstract

Myotonic dystrophy 2 (DM2) is a multisystem skeletal muscle disease caused by an expansion of tetranucleotide CCTG repeats, the transcription of which results in the accumulation of untranslated CCUG RNA. In this study, we report that CCUG repeats both bind to and misregulate the biological functions of cytoplasmic multiprotein complexes. Two CCUG-interacting complexes were subsequently purified and analyzed. A major component of one of the complexes was found to be the 20S catalytic core complex of the proteasome. The second complex was found to contain CUG triplet repeat RNA-binding protein 1 (CUGBP1) and the translation initiation factor eIF2. Consistent with the biological functions of the 20S proteasome and the CUGBP1-eIF2 complexes, the stability of short-lived proteins and the levels of the translational targets of CUGBP1 were shown to be elevated in DM2 myoblasts. We found that the overexpression of CCUG repeats in human myoblasts from unaffected patients, in C2C12 myoblasts, and in a DM2 mouse model alters protein translation and degradation, similar to the alterations observed in DM2 patients. Taken together, these findings show that RNA CCUG repeats misregulate protein turnover on both the levels of translation and proteasome-mediated protein degradation.

Original languageEnglish (US)
Pages (from-to)748-762
Number of pages15
JournalAmerican Journal of Pathology
Volume175
Issue number2
DOIs
StatePublished - Aug 2009

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Myotonic Dystrophy
Myoblasts
Protein Biosynthesis
Proteasome Endopeptidase Complex
Proteolysis
RNA
Translational Peptide Chain Initiation
Trinucleotide Repeats
Multiprotein Complexes
Peptide Initiation Factors
Untranslated RNA
Microsatellite Repeats
Catalytic Domain
Skeletal Muscle
Proteins
CELF1 Protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of RNA CCUG repeats dysregulates translation and degradation of proteins in myotonic dystrophy 2 patients. / Salisbury, Elizabeth; Schoser, Benedikt; Schneider-Gold, Christiane; Wang, Guo Li; Huichalaf, Claudia; Jin, Bingwen; Sirito, Mario; Sarkar, Partha; Krahe, Ralf; Timchenko, Nikolai A.; Timchenko, Lubov T.

In: American Journal of Pathology, Vol. 175, No. 2, 08.2009, p. 748-762.

Research output: Contribution to journalArticle

Salisbury, E, Schoser, B, Schneider-Gold, C, Wang, GL, Huichalaf, C, Jin, B, Sirito, M, Sarkar, P, Krahe, R, Timchenko, NA & Timchenko, LT 2009, 'Expression of RNA CCUG repeats dysregulates translation and degradation of proteins in myotonic dystrophy 2 patients', American Journal of Pathology, vol. 175, no. 2, pp. 748-762. https://doi.org/10.2353/ajpath.2009.090047
Salisbury, Elizabeth ; Schoser, Benedikt ; Schneider-Gold, Christiane ; Wang, Guo Li ; Huichalaf, Claudia ; Jin, Bingwen ; Sirito, Mario ; Sarkar, Partha ; Krahe, Ralf ; Timchenko, Nikolai A. ; Timchenko, Lubov T. / Expression of RNA CCUG repeats dysregulates translation and degradation of proteins in myotonic dystrophy 2 patients. In: American Journal of Pathology. 2009 ; Vol. 175, No. 2. pp. 748-762.
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