Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells

Walter H. Günzburg, Brian Salmons, Alex Schlaeffli, Sylviane Moritz-legrand, Walis Jones, Nurul H. Sarkar, Robert Ullrich

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Three carcinoma-associated oncogenes, two of which have been strongly implicated in human mammary tumorigenesis, have been introduced into a novel mouse mammary epithelial cell line, EF43, that retains many differentiated functions. The effect of oncogene expression upon classical transformation parameters as well as parameters specific for mammary epithelial cells such as growth in three-dimensional collagen matrices and the ability to repopulate the cleared mammary fat pad and to form alveolar structures in vivo has been investigated. Expression of v-myc in EF43 cells results in no obvious phenotypic changes, and does not confer tumorigenic potential upon the cells. Expression of v-Ha-ras confers upon EF43 cells the ability to grow rapidly, grow in an anchorage-independent manner, results in tumor formation in nude and syngeneic animals, abolishes their ability to repopulate the mammary gland and, instead, results in rapid induction of anaplastic tumors. The v-mil oncogene, an avian homolog of the mouse v-mht and human c-raf oncogenes, previously thought to be non-transforming in the absence of a co-operating oncogene, transforms EF43 cells, allowing them to grow in an anchorage-independent manner, form tumors in nude mice and abolishes their ability to repopulate the cleared mammary fat pad. In contrast to v-ras, however, the tumors arising from v-mil expression have a differentiated morphology, typical of adenocarcinomas. Thus, different oncogenes show varying degrees of inhibition of the differentiation of mammary epithelial cells in vivo.

Original languageEnglish (US)
Pages (from-to)1849-1856
Number of pages8
JournalCarcinogenesis
Volume9
Issue number10
DOIs
StatePublished - Oct 1988
Externally publishedYes

Fingerprint

ras Genes
Oncogenes
Tumors
Breast
Epithelial Cells
Cell
Oils and fats
Tumor
Mouse
Adipose Tissue
Neoplasms
Collagen
Animals
Human Mammary Glands
Nude Mice
Carcinogenesis
Adenocarcinoma
Proof by induction
Carcinoma
Cell Line

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Günzburg, W. H., Salmons, B., Schlaeffli, A., Moritz-legrand, S., Jones, W., Sarkar, N. H., & Ullrich, R. (1988). Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells. Carcinogenesis, 9(10), 1849-1856. https://doi.org/10.1093/carcin/9.10.1849

Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells. / Günzburg, Walter H.; Salmons, Brian; Schlaeffli, Alex; Moritz-legrand, Sylviane; Jones, Walis; Sarkar, Nurul H.; Ullrich, Robert.

In: Carcinogenesis, Vol. 9, No. 10, 10.1988, p. 1849-1856.

Research output: Contribution to journalArticle

Günzburg, WH, Salmons, B, Schlaeffli, A, Moritz-legrand, S, Jones, W, Sarkar, NH & Ullrich, R 1988, 'Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells', Carcinogenesis, vol. 9, no. 10, pp. 1849-1856. https://doi.org/10.1093/carcin/9.10.1849
Günzburg WH, Salmons B, Schlaeffli A, Moritz-legrand S, Jones W, Sarkar NH et al. Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells. Carcinogenesis. 1988 Oct;9(10):1849-1856. https://doi.org/10.1093/carcin/9.10.1849
Günzburg, Walter H. ; Salmons, Brian ; Schlaeffli, Alex ; Moritz-legrand, Sylviane ; Jones, Walis ; Sarkar, Nurul H. ; Ullrich, Robert. / Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells. In: Carcinogenesis. 1988 ; Vol. 9, No. 10. pp. 1849-1856.
@article{7e4adbff60d14c33993712a2ee8f91ea,
title = "Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells",
abstract = "Three carcinoma-associated oncogenes, two of which have been strongly implicated in human mammary tumorigenesis, have been introduced into a novel mouse mammary epithelial cell line, EF43, that retains many differentiated functions. The effect of oncogene expression upon classical transformation parameters as well as parameters specific for mammary epithelial cells such as growth in three-dimensional collagen matrices and the ability to repopulate the cleared mammary fat pad and to form alveolar structures in vivo has been investigated. Expression of v-myc in EF43 cells results in no obvious phenotypic changes, and does not confer tumorigenic potential upon the cells. Expression of v-Ha-ras confers upon EF43 cells the ability to grow rapidly, grow in an anchorage-independent manner, results in tumor formation in nude and syngeneic animals, abolishes their ability to repopulate the mammary gland and, instead, results in rapid induction of anaplastic tumors. The v-mil oncogene, an avian homolog of the mouse v-mht and human c-raf oncogenes, previously thought to be non-transforming in the absence of a co-operating oncogene, transforms EF43 cells, allowing them to grow in an anchorage-independent manner, form tumors in nude mice and abolishes their ability to repopulate the cleared mammary fat pad. In contrast to v-ras, however, the tumors arising from v-mil expression have a differentiated morphology, typical of adenocarcinomas. Thus, different oncogenes show varying degrees of inhibition of the differentiation of mammary epithelial cells in vivo.",
author = "G{\"u}nzburg, {Walter H.} and Brian Salmons and Alex Schlaeffli and Sylviane Moritz-legrand and Walis Jones and Sarkar, {Nurul H.} and Robert Ullrich",
year = "1988",
month = "10",
doi = "10.1093/carcin/9.10.1849",
language = "English (US)",
volume = "9",
pages = "1849--1856",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Expression of the oncogenes mil and ras abolishes the in vivo differentiation of mammary epithelial cells

AU - Günzburg, Walter H.

AU - Salmons, Brian

AU - Schlaeffli, Alex

AU - Moritz-legrand, Sylviane

AU - Jones, Walis

AU - Sarkar, Nurul H.

AU - Ullrich, Robert

PY - 1988/10

Y1 - 1988/10

N2 - Three carcinoma-associated oncogenes, two of which have been strongly implicated in human mammary tumorigenesis, have been introduced into a novel mouse mammary epithelial cell line, EF43, that retains many differentiated functions. The effect of oncogene expression upon classical transformation parameters as well as parameters specific for mammary epithelial cells such as growth in three-dimensional collagen matrices and the ability to repopulate the cleared mammary fat pad and to form alveolar structures in vivo has been investigated. Expression of v-myc in EF43 cells results in no obvious phenotypic changes, and does not confer tumorigenic potential upon the cells. Expression of v-Ha-ras confers upon EF43 cells the ability to grow rapidly, grow in an anchorage-independent manner, results in tumor formation in nude and syngeneic animals, abolishes their ability to repopulate the mammary gland and, instead, results in rapid induction of anaplastic tumors. The v-mil oncogene, an avian homolog of the mouse v-mht and human c-raf oncogenes, previously thought to be non-transforming in the absence of a co-operating oncogene, transforms EF43 cells, allowing them to grow in an anchorage-independent manner, form tumors in nude mice and abolishes their ability to repopulate the cleared mammary fat pad. In contrast to v-ras, however, the tumors arising from v-mil expression have a differentiated morphology, typical of adenocarcinomas. Thus, different oncogenes show varying degrees of inhibition of the differentiation of mammary epithelial cells in vivo.

AB - Three carcinoma-associated oncogenes, two of which have been strongly implicated in human mammary tumorigenesis, have been introduced into a novel mouse mammary epithelial cell line, EF43, that retains many differentiated functions. The effect of oncogene expression upon classical transformation parameters as well as parameters specific for mammary epithelial cells such as growth in three-dimensional collagen matrices and the ability to repopulate the cleared mammary fat pad and to form alveolar structures in vivo has been investigated. Expression of v-myc in EF43 cells results in no obvious phenotypic changes, and does not confer tumorigenic potential upon the cells. Expression of v-Ha-ras confers upon EF43 cells the ability to grow rapidly, grow in an anchorage-independent manner, results in tumor formation in nude and syngeneic animals, abolishes their ability to repopulate the mammary gland and, instead, results in rapid induction of anaplastic tumors. The v-mil oncogene, an avian homolog of the mouse v-mht and human c-raf oncogenes, previously thought to be non-transforming in the absence of a co-operating oncogene, transforms EF43 cells, allowing them to grow in an anchorage-independent manner, form tumors in nude mice and abolishes their ability to repopulate the cleared mammary fat pad. In contrast to v-ras, however, the tumors arising from v-mil expression have a differentiated morphology, typical of adenocarcinomas. Thus, different oncogenes show varying degrees of inhibition of the differentiation of mammary epithelial cells in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0023719907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023719907&partnerID=8YFLogxK

U2 - 10.1093/carcin/9.10.1849

DO - 10.1093/carcin/9.10.1849

M3 - Article

VL - 9

SP - 1849

EP - 1856

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 10

ER -