Expression of the programmed death ligand 1, B7-H1, on gastric epithelial cells after Helicobacter pylori exposure promotes development of CD4+ CD25+ FoxP3+ regulatory T cells

Ellen J. Beswick, Iryna Pinchuk, Soumita Das, Don W. Powell, Victor Reyes

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Abstract

During Helicobacter pylori infection, T cells are recruited to the gastric mucosa, but the host T-cell response is not sufficient to clear the infection. Some of the recruited T cells respond in a polarized manner to a Th1 response, while others become anergic. We have previously shown that T-cell anergy may be induced during infection by the interaction of T cells with B7-H1, which is up-regulated on the gastric epithelium during H. pylori infection. Recently, regulatory T (Treg) cells with a CD4+ CD25high FoxP3 + phenotype were found at an increased frequency in the gastric mucosa of biopsy specimens from H. pylori-infected patients. While Treg cells are important in maintaining tolerance, they can also suppress immune responses during infection. In this study, we examined the induction of the Treg phenotype when naive T cells were incubated with gastric epithelial cells exposed to H. pylori. The frequency of this phenotype was markedly decreased when B7-H1 was blocked with monoclonal antibodies or its expression was blocked with small interfering RNA. The functional role of these Treg cells was assessed in proliferation assays when the cells were cocultured with activated T cells, which effectively decreased proliferation of the cells.

Original languageEnglish
Pages (from-to)4334-4341
Number of pages8
JournalInfection and Immunity
Volume75
Issue number9
DOIs
StatePublished - Sep 2007

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CD80 Antigens
Regulatory T-Lymphocytes
Helicobacter pylori
Stomach
Epithelial Cells
T-Lymphocytes
Helicobacter Infections
Gastric Mucosa
Phenotype
Infection
Small Interfering RNA
Epithelium
Monoclonal Antibodies
Cell Proliferation
Biopsy

ASJC Scopus subject areas

  • Immunology

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Expression of the programmed death ligand 1, B7-H1, on gastric epithelial cells after Helicobacter pylori exposure promotes development of CD4+ CD25+ FoxP3+ regulatory T cells. / Beswick, Ellen J.; Pinchuk, Iryna; Das, Soumita; Powell, Don W.; Reyes, Victor.

In: Infection and Immunity, Vol. 75, No. 9, 09.2007, p. 4334-4341.

Research output: Contribution to journalArticle

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