Expression of the RAD1 and RAD3 genes of Saccharomyces cerevisiae is not affected by DNA damage or during the cell division cycle

Madan L. Nagpal, David R. Higgins, Satya Prakash

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The RAD1 and RAD3 genes of Saccharomyces cerevisiae are required for excision repair of UV damaged DNA. In addition, the RAD3 gene is essential since rad3 deletions are recessive lethals. We have examined the induction of the RAD1 and RAD3 genes by DNA damage and during the cell division cycle. We have made fusions of the RAD1 and RAD3 genes with the Escherichia coli lacZ gene encoding β-galactosidase. β-galactosidase activity was measured in a Rad+ yeast strain containing the RAD1-lacZ or the RAD3-lacZ fusion, either in a multicopy replicating plasmid or as a single copy integrant resulting from transformation with an integrating plasmid which transforms yeast by homologous recombination in the yeast genome. No induction of β-galactosidase activity occurred after ultraviolet light (UV) or 4-nitroquinoline-1-oxide (NQO) treatment. Haploid cells of mating type a were synchronized by treatment with α factor and β-galactosidase activity was determined during different cell cycle stages. No change in β-galactosidase activity was observed in the strain containing the RAD1-lacZ or the RAD3-lacZ fusion integrated in the yeast genome.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalMGG Molecular & General Genetics
Volume199
Issue number1
DOIs
StatePublished - Apr 1985
Externally publishedYes

Fingerprint

Galactosidases
DNA Damage
Saccharomyces cerevisiae
Cell Cycle
Yeasts
Genes
Ultraviolet Rays
Plasmids
Genome
4-Nitroquinoline-1-oxide
Lac Operon
Haploidy
Homologous Recombination
Essential Genes
DNA Repair
Escherichia coli
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Expression of the RAD1 and RAD3 genes of Saccharomyces cerevisiae is not affected by DNA damage or during the cell division cycle. / Nagpal, Madan L.; Higgins, David R.; Prakash, Satya.

In: MGG Molecular & General Genetics, Vol. 199, No. 1, 04.1985, p. 59-63.

Research output: Contribution to journalArticle

@article{fa7901cda28e496e9e89ab7158ee46ec,
title = "Expression of the RAD1 and RAD3 genes of Saccharomyces cerevisiae is not affected by DNA damage or during the cell division cycle",
abstract = "The RAD1 and RAD3 genes of Saccharomyces cerevisiae are required for excision repair of UV damaged DNA. In addition, the RAD3 gene is essential since rad3 deletions are recessive lethals. We have examined the induction of the RAD1 and RAD3 genes by DNA damage and during the cell division cycle. We have made fusions of the RAD1 and RAD3 genes with the Escherichia coli lacZ gene encoding β-galactosidase. β-galactosidase activity was measured in a Rad+ yeast strain containing the RAD1-lacZ or the RAD3-lacZ fusion, either in a multicopy replicating plasmid or as a single copy integrant resulting from transformation with an integrating plasmid which transforms yeast by homologous recombination in the yeast genome. No induction of β-galactosidase activity occurred after ultraviolet light (UV) or 4-nitroquinoline-1-oxide (NQO) treatment. Haploid cells of mating type a were synchronized by treatment with α factor and β-galactosidase activity was determined during different cell cycle stages. No change in β-galactosidase activity was observed in the strain containing the RAD1-lacZ or the RAD3-lacZ fusion integrated in the yeast genome.",
author = "Nagpal, {Madan L.} and Higgins, {David R.} and Satya Prakash",
year = "1985",
month = "4",
doi = "10.1007/BF00327510",
language = "English",
volume = "199",
pages = "59--63",
journal = "Molecular and General Genetics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Expression of the RAD1 and RAD3 genes of Saccharomyces cerevisiae is not affected by DNA damage or during the cell division cycle

AU - Nagpal, Madan L.

AU - Higgins, David R.

AU - Prakash, Satya

PY - 1985/4

Y1 - 1985/4

N2 - The RAD1 and RAD3 genes of Saccharomyces cerevisiae are required for excision repair of UV damaged DNA. In addition, the RAD3 gene is essential since rad3 deletions are recessive lethals. We have examined the induction of the RAD1 and RAD3 genes by DNA damage and during the cell division cycle. We have made fusions of the RAD1 and RAD3 genes with the Escherichia coli lacZ gene encoding β-galactosidase. β-galactosidase activity was measured in a Rad+ yeast strain containing the RAD1-lacZ or the RAD3-lacZ fusion, either in a multicopy replicating plasmid or as a single copy integrant resulting from transformation with an integrating plasmid which transforms yeast by homologous recombination in the yeast genome. No induction of β-galactosidase activity occurred after ultraviolet light (UV) or 4-nitroquinoline-1-oxide (NQO) treatment. Haploid cells of mating type a were synchronized by treatment with α factor and β-galactosidase activity was determined during different cell cycle stages. No change in β-galactosidase activity was observed in the strain containing the RAD1-lacZ or the RAD3-lacZ fusion integrated in the yeast genome.

AB - The RAD1 and RAD3 genes of Saccharomyces cerevisiae are required for excision repair of UV damaged DNA. In addition, the RAD3 gene is essential since rad3 deletions are recessive lethals. We have examined the induction of the RAD1 and RAD3 genes by DNA damage and during the cell division cycle. We have made fusions of the RAD1 and RAD3 genes with the Escherichia coli lacZ gene encoding β-galactosidase. β-galactosidase activity was measured in a Rad+ yeast strain containing the RAD1-lacZ or the RAD3-lacZ fusion, either in a multicopy replicating plasmid or as a single copy integrant resulting from transformation with an integrating plasmid which transforms yeast by homologous recombination in the yeast genome. No induction of β-galactosidase activity occurred after ultraviolet light (UV) or 4-nitroquinoline-1-oxide (NQO) treatment. Haploid cells of mating type a were synchronized by treatment with α factor and β-galactosidase activity was determined during different cell cycle stages. No change in β-galactosidase activity was observed in the strain containing the RAD1-lacZ or the RAD3-lacZ fusion integrated in the yeast genome.

UR - http://www.scopus.com/inward/record.url?scp=0021912862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021912862&partnerID=8YFLogxK

U2 - 10.1007/BF00327510

DO - 10.1007/BF00327510

M3 - Article

VL - 199

SP - 59

EP - 63

JO - Molecular and General Genetics

JF - Molecular and General Genetics

SN - 1617-4615

IS - 1

ER -