Expression of TNF-α and TNFR p55 in cultured amniochorion

S. J. Fortunato, Ramkumar Menon, K. F. Swan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

PROBLEM: Preterm labor and PROM are major complications of pregnancy. We have reported the possible role of amniochorionic membrane as it relates to the production of cytokines and the early onset of labor. Amniochorion is capable of responding to an infectious process with the production of IL-6 and IL-1β. Here we examine the expression of TNF-α and TNFR in amniochorion. METHOD: Amniochorionic membranes were collected and maintained in an organ explant system. Samples were frozen and/or fixed for RT-PCR, in situ hybridization, and immunocytochemistry. RESULTS: RT-PCR demonstrated mRNA for TNF-α and in situ hybridization localized mRNA in chorion and amnion. Immunocytochemistry demonstrated TNF-α peptide in amnion but not in chorion. Immunocytochemical localization of TNFR indicates presence of that peptide in both amnion and chorion. CONCLUSIONS: We conclude that the fetal membranes are sources of TNF-α and TNFR, supporting our previous work indicating that fetal membranes are active participants in the response to intraamniotic infection.

Original languageEnglish (US)
Pages (from-to)188-193
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume32
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Chorion
Amnion
Extraembryonic Membranes
In Situ Hybridization
Immunohistochemistry
Labor Onset
Polymerase Chain Reaction
Messenger RNA
Peptides
Membranes
Pregnancy Complications
Premature Obstetric Labor
Interleukin-1
Interleukin-6
Cytokines
Infection

Keywords

  • Amniochorion
  • Cytokines
  • Preterm labor
  • TNF-α
  • TNFR

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Expression of TNF-α and TNFR p55 in cultured amniochorion. / Fortunato, S. J.; Menon, Ramkumar; Swan, K. F.

In: American Journal of Reproductive Immunology, Vol. 32, No. 3, 1994, p. 188-193.

Research output: Contribution to journalArticle

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AB - PROBLEM: Preterm labor and PROM are major complications of pregnancy. We have reported the possible role of amniochorionic membrane as it relates to the production of cytokines and the early onset of labor. Amniochorion is capable of responding to an infectious process with the production of IL-6 and IL-1β. Here we examine the expression of TNF-α and TNFR in amniochorion. METHOD: Amniochorionic membranes were collected and maintained in an organ explant system. Samples were frozen and/or fixed for RT-PCR, in situ hybridization, and immunocytochemistry. RESULTS: RT-PCR demonstrated mRNA for TNF-α and in situ hybridization localized mRNA in chorion and amnion. Immunocytochemistry demonstrated TNF-α peptide in amnion but not in chorion. Immunocytochemical localization of TNFR indicates presence of that peptide in both amnion and chorion. CONCLUSIONS: We conclude that the fetal membranes are sources of TNF-α and TNFR, supporting our previous work indicating that fetal membranes are active participants in the response to intraamniotic infection.

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