Expression patterns of Wnt genes during development of an anterior part of the chicken eye

Valentina Fokina, Elena I. Frolova

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

To address the roles of Wnts in the development of the anterior eye, we used a chicken model to perform comprehensive expression analysis of all Wnt genes during anterior eye development. In analyzing the available genomic sequences, we found that the chicken genome encodes 18 Wnt proteins that are homologous to corresponding human and mouse proteins. The mRNA sequences for 12 chicken Wnt genes are available in GenBank, and mRNAs for six other Wnt genes (Wnt2, Wnt5b, Wnt7b, Wnt8b, Wnt9b, and Wnt16) were identified and cloned based on the homology to the genes from other species. In addition, we found that chicken Wnt3a and Wnt7b genes encode two alternative mRNA isoforms containing different first axons. Following in situ hybridization, we found that out of 18 Wnt genes, 11 genes were expressed in the anterior eye, exhibiting distinct temporal-spatial patterns. Several Wnts were expressed in the lens, including Wnt2 and Wnt2b in the anterior epithelium and Wnt5a, Wnt5b, Wnt7a, and Wnt7b in the differentiating lens fiber cells. In the cornea, we detected Wnt3a, Wnt6, and Wnt9b in the ocular surface ectoderm, including the corneal epithelium, and Wnt9a in the corneal endothelium from the onset of its differentiation. In the optic cup, Wnt2, Wnt2b, and Wnt9a were localized in the rim of the optic cup (presumptive iris), while Wnt5a and Wnt16 were detected in the ciliary epithelium/iris zone of the differentiated optic cup, and Wnt6 was expressed in the iridial mesenchyme. These data suggest that Wnt signaling might play important roles in anterior eye development.

Original languageEnglish (US)
Pages (from-to)496-505
Number of pages10
JournalDevelopmental Dynamics
Volume235
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Chickens
Genes
Iris
Lenses
Epithelium
Wnt Proteins
RNA Isoforms
Corneal Endothelium
Messenger RNA
Corneal Epithelium
Ectoderm
Nucleic Acid Databases
Mesoderm
Cornea
In Situ Hybridization
Axons
Genome
Proteins

Keywords

  • Anterior angle
  • Chicken
  • Ciliary body
  • Cornea
  • Development
  • Eye
  • In situ hybridization, iris
  • Lens
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Expression patterns of Wnt genes during development of an anterior part of the chicken eye. / Fokina, Valentina; Frolova, Elena I.

In: Developmental Dynamics, Vol. 235, No. 2, 01.02.2006, p. 496-505.

Research output: Contribution to journalArticle

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