Expression, purification, and characterization of the carboxyl-terminal region of the Na⁺/H⁺ exchanger

Keyphrases

• Ion Exchanger 100%
• Na + 100%
• Carboxyl-terminal Region 100%
• Glutathione S-transferase 33%
• Inclusion Bodies 33%
• Fusion Protein 33%
• Cytoplasmic Domain 33%
• Purified Proteins 33%
• Plasmid 16%
• Cell Culture 16%
• Proton 16%
• Escherichia Coli 16%
• Amino Acids 16%
• Helix 16%
• Regulatory Proteins 16%
• Secondary Structure 16%
• Dialysis 16%
• Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) 16%
• Cleavage Site 16%
• Gel Electrophoresis 16%
• Transmembrane Domain 16%
• Carboxy Terminal Domain 16%
• Plasma Membrane Proteins 16%
• Thrombin Cleavage 16%
• Random Coil 16%
• Final Yield 16%
• Circular Dichroism Spectroscopy 16%
• Tac Promoter 16%

Biochemistry, Genetics and Molecular Biology

• Copurification 100%
• C-Terminus 100%
• Sodium-Hydrogen Antiporter 100%
• Glutathione S-Transferase 40%
• Chimeric Protein 40%
• Plasmid 20%
• Promoter Region 20%
• Cell Culture 20%
• Cell Membrane 20%
• Amino Acids 20%
• Escherichia coli 20%
• Membrane Protein 20%
• Protein Secondary Structure 20%
• Polyacrylamide Gel Electrophoresis 20%
• Circular Dichroism 20%
• Protein L 20%
• Gel Electrophoresis 20%
• Thrombin 20%