External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry

Michael W. Senko, Christopher L. Hendrickson, Mark Emmett, Stone D H Shi, Alan G. Marshall

Research output: Contribution to journalArticle

424 Citations (Scopus)

Abstract

Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily by pulsed gas assisted accumulation and the incompatibility of the associated pump-down time with the frequent ion beam sampling requirement of on-line chromatographic separation. Here we describe numerous analytical advantages that accrue by trapping ions at high pressure in the first rf-only octupole of a dual octupole ion injection system before ion transfer to the ion trap in the center of the magnet for high performance mass analysis at low pressure. The new configuration improves the duty cycle for analysis of continuously generated ions, and is thus ideally suited for on-line chromatographic applications. LC/ESI FTICR MS is demonstrated on a mixture of 500 fmol of each of three peptides. Additional improvements include a fivefold increase in signal-to-noise ratio and resolving power compared to prior methods on our instrument.

Original languageEnglish (US)
Pages (from-to)970-976
Number of pages7
JournalJournal of the American Society for Mass Spectrometry
Volume8
Issue number9
DOIs
StatePublished - Sep 1997
Externally publishedYes

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Cyclotrons
Cyclotron resonance
Electrospray ionization
Fourier Analysis
Mass spectrometry
Mass Spectrometry
Fourier transforms
Ions
Liquid chromatography
Optical resolving power
Liquid Chromatography
Capillary electrophoresis
Pressure
Magnets
Capillary Electrophoresis
Signal-To-Noise Ratio
Ion beams
Signal to noise ratio
Gases
Pumps

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. / Senko, Michael W.; Hendrickson, Christopher L.; Emmett, Mark; Shi, Stone D H; Marshall, Alan G.

In: Journal of the American Society for Mass Spectrometry, Vol. 8, No. 9, 09.1997, p. 970-976.

Research output: Contribution to journalArticle

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