Abstract
Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily by pulsed gas assisted accumulation and the incompatibility of the associated pump-down time with the frequent ion beam sampling requirement of on-line chromatographic separation. Here we describe numerous analytical advantages that accrue by trapping ions at high pressure in the first rf-only octupole of a dual octupole ion injection system before ion transfer to the ion trap in the center of the magnet for high performance mass analysis at low pressure. The new configuration improves the duty cycle for analysis of continuously generated ions, and is thus ideally suited for on-line chromatographic applications. LC/ESI FTICR MS is demonstrated on a mixture of 500 fmol of each of three peptides. Additional improvements include a fivefold increase in signal-to-noise ratio and resolving power compared to prior methods on our instrument.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 970-976 |
| Number of pages | 7 |
| Journal | Journal of the American Society for Mass Spectrometry |
| Volume | 8 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Structural Biology
- Spectroscopy
Cite this
External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. / Senko, Michael W.; Hendrickson, Christopher L.; Emmett, Mark; Shi, Stone D H; Marshall, Alan G.
In: Journal of the American Society for Mass Spectrometry, Vol. 8, No. 9, 09.1997, p. 970-976.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry
AU - Senko, Michael W.
AU - Hendrickson, Christopher L.
AU - Emmett, Mark
AU - Shi, Stone D H
AU - Marshall, Alan G.
PY - 1997/9
Y1 - 1997/9
N2 - Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily by pulsed gas assisted accumulation and the incompatibility of the associated pump-down time with the frequent ion beam sampling requirement of on-line chromatographic separation. Here we describe numerous analytical advantages that accrue by trapping ions at high pressure in the first rf-only octupole of a dual octupole ion injection system before ion transfer to the ion trap in the center of the magnet for high performance mass analysis at low pressure. The new configuration improves the duty cycle for analysis of continuously generated ions, and is thus ideally suited for on-line chromatographic applications. LC/ESI FTICR MS is demonstrated on a mixture of 500 fmol of each of three peptides. Additional improvements include a fivefold increase in signal-to-noise ratio and resolving power compared to prior methods on our instrument.
AB - Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily by pulsed gas assisted accumulation and the incompatibility of the associated pump-down time with the frequent ion beam sampling requirement of on-line chromatographic separation. Here we describe numerous analytical advantages that accrue by trapping ions at high pressure in the first rf-only octupole of a dual octupole ion injection system before ion transfer to the ion trap in the center of the magnet for high performance mass analysis at low pressure. The new configuration improves the duty cycle for analysis of continuously generated ions, and is thus ideally suited for on-line chromatographic applications. LC/ESI FTICR MS is demonstrated on a mixture of 500 fmol of each of three peptides. Additional improvements include a fivefold increase in signal-to-noise ratio and resolving power compared to prior methods on our instrument.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031239787&partnerID=8YFLogxK
U2 - 10.1016/S1044-0305(97)00126-8
DO - 10.1016/S1044-0305(97)00126-8
M3 - Article
VL - 8
SP - 970
EP - 976
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 9
ER -