External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts

Ronald Tilton, K. B. Larson, J. R. Udell, B. E. Sobel, J. R. Williamson

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

To define relationships better between the duration of severe ischemia and microvascular functional integrity with an approach potentially applicable to studies in vivo, the effects of 30 and 60 minutes of global, no-flow ischemia on the coronary vasculature of isolated, perfused rabbit hearts were determined. Residue-detection data, analyzed with a two-compartment model, were used to estimate indices of microvascular function, including the mean-transit time (t̄(BSA)) of radiolabeled bovine serum albumin ( 125I-BSA), vascular into extravascular space clearance, and vascular and extravascular space volumes. It was shown that the Central Volume Principle of tracer kinetics does not hold when transport of label between vascular and extravascular spaces takes place convectively by solvent drag, and a more general expression for t̄(BSA) was derived and applied. Left ventricular end-diastolic pressure and left ventricular developed pressure were monitored with an isovolumic balloon. Aortic perfusion pressure, left ventricular end-diastolic pressure, left ventricular developed pressure and vascular space volume remained constant, while mean transit time, vascular into extravascular space clearance and extravascular space volumes increased gradually during 3-hour control perfusions. Perfusion pressure, mean transit time and extravascular space clearance increased significantly with reperfusion after 30 minutes of ischemia even though left ventricular end-diastolic and left ventricular-developed pressures returned to control levels. Vascular space volumes increased minimally, whereas extravascular space volumes increased 5-fold during reperfusion. These changes in 125I-BSA washout and permeation across endothelium with reperfusion after no-flow ischemia indicate that compromised vascular integrity is an early manifestation of ischemia with functional consequences that persist even after ischemia sufficiently brief to permit restoration of left ventricular performance.

Original languageEnglish (US)
Pages (from-to)210-225
Number of pages16
JournalCirculation Research
Volume52
Issue number2
StatePublished - 1983
Externally publishedYes

Fingerprint

Reperfusion
Blood Vessels
Ischemia
Rabbits
Ventricular Pressure
Perfusion
Blood Pressure
Bovine Serum Albumin
Endothelium
Arterial Pressure
Pressure

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Tilton, R., Larson, K. B., Udell, J. R., Sobel, B. E., & Williamson, J. R. (1983). External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts. Circulation Research, 52(2), 210-225.

External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts. / Tilton, Ronald; Larson, K. B.; Udell, J. R.; Sobel, B. E.; Williamson, J. R.

In: Circulation Research, Vol. 52, No. 2, 1983, p. 210-225.

Research output: Contribution to journalArticle

Tilton, R, Larson, KB, Udell, JR, Sobel, BE & Williamson, JR 1983, 'External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts', Circulation Research, vol. 52, no. 2, pp. 210-225.
Tilton, Ronald ; Larson, K. B. ; Udell, J. R. ; Sobel, B. E. ; Williamson, J. R. / External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts. In: Circulation Research. 1983 ; Vol. 52, No. 2. pp. 210-225.
@article{a99e134f29fa4f4e9ffe55c9a979888f,
title = "External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts",
abstract = "To define relationships better between the duration of severe ischemia and microvascular functional integrity with an approach potentially applicable to studies in vivo, the effects of 30 and 60 minutes of global, no-flow ischemia on the coronary vasculature of isolated, perfused rabbit hearts were determined. Residue-detection data, analyzed with a two-compartment model, were used to estimate indices of microvascular function, including the mean-transit time (t̄(BSA)) of radiolabeled bovine serum albumin ( 125I-BSA), vascular into extravascular space clearance, and vascular and extravascular space volumes. It was shown that the Central Volume Principle of tracer kinetics does not hold when transport of label between vascular and extravascular spaces takes place convectively by solvent drag, and a more general expression for t̄(BSA) was derived and applied. Left ventricular end-diastolic pressure and left ventricular developed pressure were monitored with an isovolumic balloon. Aortic perfusion pressure, left ventricular end-diastolic pressure, left ventricular developed pressure and vascular space volume remained constant, while mean transit time, vascular into extravascular space clearance and extravascular space volumes increased gradually during 3-hour control perfusions. Perfusion pressure, mean transit time and extravascular space clearance increased significantly with reperfusion after 30 minutes of ischemia even though left ventricular end-diastolic and left ventricular-developed pressures returned to control levels. Vascular space volumes increased minimally, whereas extravascular space volumes increased 5-fold during reperfusion. These changes in 125I-BSA washout and permeation across endothelium with reperfusion after no-flow ischemia indicate that compromised vascular integrity is an early manifestation of ischemia with functional consequences that persist even after ischemia sufficiently brief to permit restoration of left ventricular performance.",
author = "Ronald Tilton and Larson, {K. B.} and Udell, {J. R.} and Sobel, {B. E.} and Williamson, {J. R.}",
year = "1983",
language = "English (US)",
volume = "52",
pages = "210--225",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - External detection of early microvascular dysfunction after no-flow ischemia followed by reperfusion in isolated rabbit hearts

AU - Tilton, Ronald

AU - Larson, K. B.

AU - Udell, J. R.

AU - Sobel, B. E.

AU - Williamson, J. R.

PY - 1983

Y1 - 1983

N2 - To define relationships better between the duration of severe ischemia and microvascular functional integrity with an approach potentially applicable to studies in vivo, the effects of 30 and 60 minutes of global, no-flow ischemia on the coronary vasculature of isolated, perfused rabbit hearts were determined. Residue-detection data, analyzed with a two-compartment model, were used to estimate indices of microvascular function, including the mean-transit time (t̄(BSA)) of radiolabeled bovine serum albumin ( 125I-BSA), vascular into extravascular space clearance, and vascular and extravascular space volumes. It was shown that the Central Volume Principle of tracer kinetics does not hold when transport of label between vascular and extravascular spaces takes place convectively by solvent drag, and a more general expression for t̄(BSA) was derived and applied. Left ventricular end-diastolic pressure and left ventricular developed pressure were monitored with an isovolumic balloon. Aortic perfusion pressure, left ventricular end-diastolic pressure, left ventricular developed pressure and vascular space volume remained constant, while mean transit time, vascular into extravascular space clearance and extravascular space volumes increased gradually during 3-hour control perfusions. Perfusion pressure, mean transit time and extravascular space clearance increased significantly with reperfusion after 30 minutes of ischemia even though left ventricular end-diastolic and left ventricular-developed pressures returned to control levels. Vascular space volumes increased minimally, whereas extravascular space volumes increased 5-fold during reperfusion. These changes in 125I-BSA washout and permeation across endothelium with reperfusion after no-flow ischemia indicate that compromised vascular integrity is an early manifestation of ischemia with functional consequences that persist even after ischemia sufficiently brief to permit restoration of left ventricular performance.

AB - To define relationships better between the duration of severe ischemia and microvascular functional integrity with an approach potentially applicable to studies in vivo, the effects of 30 and 60 minutes of global, no-flow ischemia on the coronary vasculature of isolated, perfused rabbit hearts were determined. Residue-detection data, analyzed with a two-compartment model, were used to estimate indices of microvascular function, including the mean-transit time (t̄(BSA)) of radiolabeled bovine serum albumin ( 125I-BSA), vascular into extravascular space clearance, and vascular and extravascular space volumes. It was shown that the Central Volume Principle of tracer kinetics does not hold when transport of label between vascular and extravascular spaces takes place convectively by solvent drag, and a more general expression for t̄(BSA) was derived and applied. Left ventricular end-diastolic pressure and left ventricular developed pressure were monitored with an isovolumic balloon. Aortic perfusion pressure, left ventricular end-diastolic pressure, left ventricular developed pressure and vascular space volume remained constant, while mean transit time, vascular into extravascular space clearance and extravascular space volumes increased gradually during 3-hour control perfusions. Perfusion pressure, mean transit time and extravascular space clearance increased significantly with reperfusion after 30 minutes of ischemia even though left ventricular end-diastolic and left ventricular-developed pressures returned to control levels. Vascular space volumes increased minimally, whereas extravascular space volumes increased 5-fold during reperfusion. These changes in 125I-BSA washout and permeation across endothelium with reperfusion after no-flow ischemia indicate that compromised vascular integrity is an early manifestation of ischemia with functional consequences that persist even after ischemia sufficiently brief to permit restoration of left ventricular performance.

UR - http://www.scopus.com/inward/record.url?scp=0020655434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020655434&partnerID=8YFLogxK

M3 - Article

C2 - 6825215

AN - SCOPUS:0020655434

VL - 52

SP - 210

EP - 225

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 2

ER -