Extinction of alpha-fetoprotein gene expression in somatic cell hybrids involves cis-acting DNA elements.

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14 Citations (Scopus)

Abstract

alpha-Fetoprotein (AFP), a liver-specific protein, is extinguished in somatic cell hybrids formed by the fusion of mouse hepatoma cells (BWTG3) with rat fibroblast cells (JF1). Our studies show that the extinction of mouse AFP expression in these somatic cell hybrids may involve at least two cis-acting regulatory domains, i.e., the enhancer elements and a tissue-specific promoter region, which are located in the 5'-flanking region of the AFP gene.

Original languageEnglish (US)
Pages (from-to)2606-2609
Number of pages4
JournalMolecular and Cellular Biology
Volume7
Issue number7
StatePublished - Jul 1987
Externally publishedYes

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Hybrid Cells
alpha-Fetoproteins
Gene Expression
DNA
5' Flanking Region
Genetic Promoter Regions
Hepatocellular Carcinoma
Fibroblasts
Liver
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

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title = "Extinction of alpha-fetoprotein gene expression in somatic cell hybrids involves cis-acting DNA elements.",
abstract = "alpha-Fetoprotein (AFP), a liver-specific protein, is extinguished in somatic cell hybrids formed by the fusion of mouse hepatoma cells (BWTG3) with rat fibroblast cells (JF1). Our studies show that the extinction of mouse AFP expression in these somatic cell hybrids may involve at least two cis-acting regulatory domains, i.e., the enhancer elements and a tissue-specific promoter region, which are located in the 5'-flanking region of the AFP gene.",
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