Extracellular hydrolytic enzymes of rabbit dermal tuberculous lesions and tuberculin reactions collected in skin chambers

M. Sugimoto, A. M. Dannenberg, L. M. Wahl, W. H. Ettinger, A. T. Hastie, D. C. Daniels, Christopher Thomas, L. Demoulin-Brahy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To evaluate extracellular hydrolytic enzymes in an in vivo system, plastic chambers were glued over rabbit dermal BCG lesions in various stages of development, after the central epithelium was removed with a scalpel. They were filled with tissue culture medium and left in place 2 days. The following enzymes in the fluid were assayed: collagenase (an enzyme secreted but not stored in macrophages); lysozyme (both secreted and stored); DNase and RNase (released on cell death and possibly regurgitated but not secreted); and, as a control, lactic dehydrogenase (released only on cell death). Tissue sections were prepared and studied histologically for the type of cell infiltrate, for β-galactosidase (a marker enzyme for macrophage activation), and for necrosis. At 11 and 18 days of age the BCG lesions were largest and the number of activated macrophages in the chamber beds was highest. At this time the levels of the 5 enzymes assayed in the chamber fluids reached their peaks, tuberculin hypersensitivity was well developed, and the bacilli components would still be plentiful. In general, the chamber fluids from 11- and 18-day BCG lesions contained higher enzyme levels than chamber fluids from tuberculin reactions. Active collagenase was only detected in fluids from such BCG lesions. Evidently, the serum in the chamber fluids was sufficient to inhibit the lower amounts of collagenase probably released from smaller BCG lesions and tuberculin reactions (and from the 2-week polystyrene lesions that were also evaluated). These studies demonstrate that in chronic inflammatory reactions, both acid-acting and neutral-acting hydrolytic enzymes are released extracellularly. Tissue components would be hydrolyzed locally wherever the acid-acting hydrolytic enzymes encounter a drop in pH and wherever the concentration of neutral-acting hydrolytic enzymes exceeds the concentration of their inhibitors.

Original languageEnglish (US)
Pages (from-to)583-607
Number of pages25
JournalAmerican Journal of Pathology
Volume90
Issue number3
StatePublished - 1978
Externally publishedYes

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Tuberculin
Rabbits
Mycobacterium bovis
Skin
Enzymes
Collagenases
Cell Death
Macrophages
Galactosidases
Acids
Enzyme Activation
Deoxyribonucleases
Macrophage Activation
Polystyrenes
Ribonucleases
Muramidase
Bacillus
Plastics
Culture Media
Oxidoreductases

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sugimoto, M., Dannenberg, A. M., Wahl, L. M., Ettinger, W. H., Hastie, A. T., Daniels, D. C., ... Demoulin-Brahy, L. (1978). Extracellular hydrolytic enzymes of rabbit dermal tuberculous lesions and tuberculin reactions collected in skin chambers. American Journal of Pathology, 90(3), 583-607.

Extracellular hydrolytic enzymes of rabbit dermal tuberculous lesions and tuberculin reactions collected in skin chambers. / Sugimoto, M.; Dannenberg, A. M.; Wahl, L. M.; Ettinger, W. H.; Hastie, A. T.; Daniels, D. C.; Thomas, Christopher; Demoulin-Brahy, L.

In: American Journal of Pathology, Vol. 90, No. 3, 1978, p. 583-607.

Research output: Contribution to journalArticle

Sugimoto, M, Dannenberg, AM, Wahl, LM, Ettinger, WH, Hastie, AT, Daniels, DC, Thomas, C & Demoulin-Brahy, L 1978, 'Extracellular hydrolytic enzymes of rabbit dermal tuberculous lesions and tuberculin reactions collected in skin chambers', American Journal of Pathology, vol. 90, no. 3, pp. 583-607.
Sugimoto M, Dannenberg AM, Wahl LM, Ettinger WH, Hastie AT, Daniels DC et al. Extracellular hydrolytic enzymes of rabbit dermal tuberculous lesions and tuberculin reactions collected in skin chambers. American Journal of Pathology. 1978;90(3):583-607.
Sugimoto, M. ; Dannenberg, A. M. ; Wahl, L. M. ; Ettinger, W. H. ; Hastie, A. T. ; Daniels, D. C. ; Thomas, Christopher ; Demoulin-Brahy, L. / Extracellular hydrolytic enzymes of rabbit dermal tuberculous lesions and tuberculin reactions collected in skin chambers. In: American Journal of Pathology. 1978 ; Vol. 90, No. 3. pp. 583-607.
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AU - Hastie, A. T.

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AU - Thomas, Christopher

AU - Demoulin-Brahy, L.

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