Abstract
To move forward, migrating cells must generate traction forces through surface receptors bound to extracellular matrix molecules coupled to a rigid structure. We investigated whether cells sample and respond to the rigidity of the anchoring matrix. Movement of beads coated with fibronectin or an anti-integrin antibody was restrained with an optical trap on fibroblasts to mimic extracellular attachment sites of different resistance. Cells precisely sense the restraining force on fibronectin beads and respond by a localized, proportional strengthening of the cytoskeleton linkages, allowing stronger force to be exerted on the integrins. This strengthening was absent or transient with antibody beads, but restored with soluble fibronectin. Hence, ligand binding site occupancy was required. Finally, phenylarsine oxide inhibited strengthening of cytoskeletal linkages, indicating a role for dephosphorylation. Thus, the strength of integrin-cytoskeleton linkages is dependent on matrix rigidity and on its biochemical composition. Matrix rigidity may, therefore, serve as a guidance cue in a process of mechanotaxis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 39-48 |
| Number of pages | 10 |
| Journal | Cell |
| Volume | 88 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 10 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Extracellular matrix rigidity causes strengthening of integrin- cytoskeleton linkages. / Choquet, Daniel; Felsenfeld, Dan P.; Sheetz, Michael.
In: Cell, Vol. 88, No. 1, 10.01.1997, p. 39-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extracellular matrix rigidity causes strengthening of integrin- cytoskeleton linkages
AU - Choquet, Daniel
AU - Felsenfeld, Dan P.
AU - Sheetz, Michael
PY - 1997/1/10
Y1 - 1997/1/10
N2 - To move forward, migrating cells must generate traction forces through surface receptors bound to extracellular matrix molecules coupled to a rigid structure. We investigated whether cells sample and respond to the rigidity of the anchoring matrix. Movement of beads coated with fibronectin or an anti-integrin antibody was restrained with an optical trap on fibroblasts to mimic extracellular attachment sites of different resistance. Cells precisely sense the restraining force on fibronectin beads and respond by a localized, proportional strengthening of the cytoskeleton linkages, allowing stronger force to be exerted on the integrins. This strengthening was absent or transient with antibody beads, but restored with soluble fibronectin. Hence, ligand binding site occupancy was required. Finally, phenylarsine oxide inhibited strengthening of cytoskeletal linkages, indicating a role for dephosphorylation. Thus, the strength of integrin-cytoskeleton linkages is dependent on matrix rigidity and on its biochemical composition. Matrix rigidity may, therefore, serve as a guidance cue in a process of mechanotaxis.
AB - To move forward, migrating cells must generate traction forces through surface receptors bound to extracellular matrix molecules coupled to a rigid structure. We investigated whether cells sample and respond to the rigidity of the anchoring matrix. Movement of beads coated with fibronectin or an anti-integrin antibody was restrained with an optical trap on fibroblasts to mimic extracellular attachment sites of different resistance. Cells precisely sense the restraining force on fibronectin beads and respond by a localized, proportional strengthening of the cytoskeleton linkages, allowing stronger force to be exerted on the integrins. This strengthening was absent or transient with antibody beads, but restored with soluble fibronectin. Hence, ligand binding site occupancy was required. Finally, phenylarsine oxide inhibited strengthening of cytoskeletal linkages, indicating a role for dephosphorylation. Thus, the strength of integrin-cytoskeleton linkages is dependent on matrix rigidity and on its biochemical composition. Matrix rigidity may, therefore, serve as a guidance cue in a process of mechanotaxis.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030994017&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81856-5
DO - 10.1016/S0092-8674(00)81856-5
M3 - Article
C2 - 9019403
AN - SCOPUS:0030994017
VL - 88
SP - 39
EP - 48
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -