Extracellular vesicles mediate receptor-independent transmission of novel tick-borne bunyavirus

Jesus A. Silvas, Vsevolod Popov, Adriana Paulucci-Holthauzen, Patricia Aguilar

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) virus is a newly recognized member of the genus Phlebovirus in the family Bunyaviridae. The virus was isolated from patients presenting with hemorrhagic manifestations and an initial case fatality rate of 12 to 30% was reported. Due to the recent emergence of this pathogen, there is limited knowledge on the molecular virology of SFTS virus. Recently, we reported that the SFTS virus NSs protein inhibited the activation of the beta interferon (IFN-β) promoter. Furthermore, we also found that SFTS virus NSs relocalizes key components of the IFN response into NSs-induced cytoplasmic structures. Due to the important role these structures play during SFTS virus replication, we conducted live cell imaging studies to gain further insight into the role and trafficking of these cytoplasmic structures during virus infection. We found that some of the SFTS virus NSs-positive cytoplasmic structures were secreted to the extracellular space and endocytosed by neighboring cells. We also found that these secreted structures isolated from NSs-expressing cells and SFTS virus-infected cells were positive for the viral protein NSs and the host protein CD63, a protein associated with extracellular vesicles. Electron microscopy studies also revealed that the isolated CD63-immunoprecipitated extracellular vesicles produced during SFTS virus infection contained virions. The virions harbored within these structures were efficiently delivered to uninfected cells and were able to sustain SFTS virus replication. Altogether, these results suggest that SFTS virus exploits extracellular vesicles to mediate virus receptor-independent transmission to host cells and open the avenue for novel therapeutic strategies against SFTS virus and related pathogens.

Original languageEnglish (US)
Pages (from-to)873-886
Number of pages14
JournalJournal of Virology
Volume90
Issue number2
DOIs
StatePublished - 2016

Fingerprint

Orthobunyavirus
thrombocytopenia
Ticks
Thrombocytopenia
fever
ticks
Fever
receptors
viruses
Viruses
Cytoplasmic Structures
Virus Diseases
cells
Virus Replication
virus replication
virion
Virion
Phlebovirus
Extracellular Vesicles
Viral Nonstructural Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Extracellular vesicles mediate receptor-independent transmission of novel tick-borne bunyavirus. / Silvas, Jesus A.; Popov, Vsevolod; Paulucci-Holthauzen, Adriana; Aguilar, Patricia.

In: Journal of Virology, Vol. 90, No. 2, 2016, p. 873-886.

Research output: Contribution to journalArticle

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