Fabrication of elastomer pillar arrays with modulated stiffness for cellular force measurements

S. Ghassemi; N. Biais; K. Maniura; S. J. Wind; M. P. Sheetz; J. Hone

doi:10.1116/1.3013424

Fabrication of elastomer pillar arrays with modulated stiffness for cellular force measurements

S. Ghassemi, N. Biais, K. Maniura, S. J. Wind, M. P. Sheetz, J. Hone

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The mechanical properties of a cell's environment can alter behavior such as migration and spreading, and control the differentiation path of stem cells. Here the authors describe a technique for fabricating substrates whose rigidity can be controlled locally without altering the contact area for cell spreading. The substrates consist of elastomeric pillar arrays in which the top surface is uniform but the pillar height is changed across a sharp step. Preliminary results demonstrate the effects on cell migration and morphology at the step boundary.

Original language	English (US)
Pages (from-to)	2549-2553
Number of pages	5
Journal	Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
Volume	26
Issue number	6
DOIs	https://doi.org/10.1116/1.3013424
State	Published - 2008
Externally published	Yes

ASJC Scopus subject areas

Condensed Matter Physics
Electrical and Electronic Engineering

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Cite this

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title = "Fabrication of elastomer pillar arrays with modulated stiffness for cellular force measurements",

abstract = "The mechanical properties of a cell's environment can alter behavior such as migration and spreading, and control the differentiation path of stem cells. Here the authors describe a technique for fabricating substrates whose rigidity can be controlled locally without altering the contact area for cell spreading. The substrates consist of elastomeric pillar arrays in which the top surface is uniform but the pillar height is changed across a sharp step. Preliminary results demonstrate the effects on cell migration and morphology at the step boundary.",

author = "S. Ghassemi and N. Biais and K. Maniura and Wind, {S. J.} and Sheetz, {M. P.} and J. Hone",

note = "Funding Information: This work was performed in part at the Cornell NanoScale Facility, a member of the National Nanotechnology Infrastructure Network, which is supported by the National Science Foundation (Grant No. ECS-0335765). This publication and the project described were supported by the National Institutes of Health through the NIH Roadmap for Medical Research (PN2 EY016586). Mesenchymal stem cells were a kind gift of Junya Toguchida (Kyoto University, Japan)",

year = "2008",

doi = "10.1116/1.3013424",

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T1 - Fabrication of elastomer pillar arrays with modulated stiffness for cellular force measurements

AU - Ghassemi, S.

AU - Biais, N.

AU - Maniura, K.

AU - Wind, S. J.

AU - Sheetz, M. P.

AU - Hone, J.

N1 - Funding Information: This work was performed in part at the Cornell NanoScale Facility, a member of the National Nanotechnology Infrastructure Network, which is supported by the National Science Foundation (Grant No. ECS-0335765). This publication and the project described were supported by the National Institutes of Health through the NIH Roadmap for Medical Research (PN2 EY016586). Mesenchymal stem cells were a kind gift of Junya Toguchida (Kyoto University, Japan)

PY - 2008

Y1 - 2008

N2 - The mechanical properties of a cell's environment can alter behavior such as migration and spreading, and control the differentiation path of stem cells. Here the authors describe a technique for fabricating substrates whose rigidity can be controlled locally without altering the contact area for cell spreading. The substrates consist of elastomeric pillar arrays in which the top surface is uniform but the pillar height is changed across a sharp step. Preliminary results demonstrate the effects on cell migration and morphology at the step boundary.

AB - The mechanical properties of a cell's environment can alter behavior such as migration and spreading, and control the differentiation path of stem cells. Here the authors describe a technique for fabricating substrates whose rigidity can be controlled locally without altering the contact area for cell spreading. The substrates consist of elastomeric pillar arrays in which the top surface is uniform but the pillar height is changed across a sharp step. Preliminary results demonstrate the effects on cell migration and morphology at the step boundary.

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Fabrication of elastomer pillar arrays with modulated stiffness for cellular force measurements

Abstract

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