Facilitation of NMDA-induced currents and Ca2+ transients in the rat substantia gelatinosa neurons after ligation of L5-L6 spinal nerves

Dmitri Isaev, Gabor Gerber, Soon Kwon Park, Jin Chung, Mirjana Randi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

This study employing a rodent model of neuropathic pain investigated the influence of partial nerve injury on the ability of NMDA receptor activation to induce membrane currents and rises in cytosolic concentration of free calcium ([Ca2+]i) in the rat substantia gelatinosa (SG) neurons using simultaneous whole-cell patch-clamp recording and fura-2 calcium imaging in spinal slices. The novel findings are that: (1) L5-L6 spinal nerve ligation produces a sustained facilitation of NMDA-mediated membrane currents and [Ca2+]i rises both in the soma and dendrites of SG neurons on the injured side on post-operative days 4-13 after injury. (2) It appears that SG neurons in slices from injured rats recover from Ca2+ load less efficiently than neurons from naive rats. (3) The membrane depolarization-induced Ca2+ transients in SG neurons are not modified following spinal nerve ligation. The temporal profile of the changes in Ca2+ transients correlated well with the development of mechanical and thermal allodynia and hyperalgesia. These results suggest an important role of NMDA-mediated calcium signalling in the pathogenesis of neuropathic pain following spinal nerve injury.

Original languageEnglish (US)
Pages (from-to)4055-4061
Number of pages7
JournalNeuroReport
Volume11
Issue number18
StatePublished - 2000
Externally publishedYes

Fingerprint

Substantia Gelatinosa
Spinal Nerves
N-Methylaspartate
Hyperalgesia
Ligation
Neurons
Neuralgia
Membranes
Calcium
Spinal Injuries
Calcium Signaling
Fura-2
Wounds and Injuries
Carisoprodol
Dendrites
N-Methyl-D-Aspartate Receptors
Rodentia

Keywords

  • Intracellular calcium
  • Nerve injury
  • NMDA
  • Spinal slice
  • Substantia gelatinosa

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Facilitation of NMDA-induced currents and Ca2+ transients in the rat substantia gelatinosa neurons after ligation of L5-L6 spinal nerves. / Isaev, Dmitri; Gerber, Gabor; Park, Soon Kwon; Chung, Jin; Randi, Mirjana.

In: NeuroReport, Vol. 11, No. 18, 2000, p. 4055-4061.

Research output: Contribution to journalArticle

Isaev, Dmitri ; Gerber, Gabor ; Park, Soon Kwon ; Chung, Jin ; Randi, Mirjana. / Facilitation of NMDA-induced currents and Ca2+ transients in the rat substantia gelatinosa neurons after ligation of L5-L6 spinal nerves. In: NeuroReport. 2000 ; Vol. 11, No. 18. pp. 4055-4061.
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