False positive ultrasensitive HIV bDNA viral load results in diagnosis of perinatal HIV-infection in the era of low transmission

Janak Patel, Erika Anderson, Jianli Dong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The performance of ultrasensitive, quantitative assays in the diagnosis of perinatal HIV has not been documented in a population with low perinatal transmission due to use of antiretroviral therapy. Methods: We studied 205 plasma samples from 71 perinatally-exposed infants for HIV diagnosis using a quantitative branched DNA (bDNA) assay with a detection range of 75-500,000 copies/mL. Results: Twelve (16.9%) infants had at least 1 positive bDNA result. Two (2.8%) infants had confirmed HIV-infections as determined by ≥2 subsequent positive bDNA results (range=1,818-500,000 copies/mL). The remaining 10 (14.5%) infants had 1 positive bDNA test (range=81-440 copies/mL) with 2 subsequent negative bDNA tests and/or a negative HIV ELISA test; these infants were considered to have a false-positive result. The performance of internal assay standards was within acceptable limits. Conclusions: Our study suggests caution is needed in the interpretation of single, low-level, positive results of the ultrasensitive, quantitative bDNA test for HIV diagnosis of perinatallyexposed infants.

Original languageEnglish (US)
Pages (from-to)611-614
Number of pages4
JournalLaboratory Medicine
Volume40
Issue number10
DOIs
StatePublished - Oct 2009

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Viral DNA
Viral Load
HIV Infections
HIV
DNA
Assays
Branched DNA Signal Amplification Assay
Enzyme-Linked Immunosorbent Assay
Plasmas
Population

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

False positive ultrasensitive HIV bDNA viral load results in diagnosis of perinatal HIV-infection in the era of low transmission. / Patel, Janak; Anderson, Erika; Dong, Jianli.

In: Laboratory Medicine, Vol. 40, No. 10, 10.2009, p. 611-614.

Research output: Contribution to journalArticle

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