Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia

Kaw Bing Chua, Khean Jin Goh, Kum Thong Wong, Adeeba Kamarulzaman, Patrick Seow Koon Tan, Thomas G. Ksiazek, Sherif R. Zaki, George Paul, Sai Kit Lam, Chong Tin Tan

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486 Scopus citations

Abstract

Background. Between February and April, 1999, an outbreak of viral encephalitis occurred among pig-farmers in Malaysia. We report findings for the first three patients who died. Methods. Samples of tissue were taken at necropsy. Blood and cerebrospinal-fluid (CSF) samples taken before death were cultured for viruses, and tested for antibodies to viruses. Findings. The three pig-farmers presented with fever, headache, and altered level of consciousness. Myoclonus was present in two patients. There were signs of brainstem dysfunction with hypertension and tachycardia. Rapid deterioration led to irreversible hypotension and death. A virus causing syncytial formation of vero cells was cultured from the CSF of two patients after 5 days; the virus stained positively with antibodies against Hendra virus by indirect immunofluorescence. IgM capture ELISA showed that all three patients had IgM antibodies in CSF against Hendra viral antigens. Necropsy showed widespread microinfarction in the central nervous system and other organs resulting from vasculitis-induced thrombosis. There was no clinical evidence of pulmonary involvement. Inclusion bodies likely to be of viral origin were noted in neurons near vasculitic blood vessels. Interpretation. The causative agent was a previously undescribed paramyxovirus related to the Hendra virus. Close contact with infected pigs may be the source of the viral transmission. Clinically and epidemiologically the infection is distinct from infection by the Hendra virus. We propose that this Hendra-like virus was the cause of the outbreak of encephalitis in Malaysia.

Original languageEnglish (US)
Pages (from-to)1257-1259
Number of pages3
JournalLancet
Volume354
Issue number9186
DOIs
StatePublished - Oct 9 1999
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Chua, K. B., Goh, K. J., Wong, K. T., Kamarulzaman, A., Tan, P. S. K., Ksiazek, T. G., Zaki, S. R., Paul, G., Lam, S. K., & Tan, C. T. (1999). Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia. Lancet, 354(9186), 1257-1259. https://doi.org/10.1016/S0140-6736(99)04299-3