Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei

William J. Durham, Yi Ping Li, Eric Gerken, Mehran Farid, Sandrine Arbogast, Robert R. Wolfe, Michael B. Reid

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

This study tested the hypothesis that skeletal muscle contraction activates nuclear factor-κB (NF-κB), a putative regulator of muscle protein breakdown. Muscle biopsies were obtained from the vastus lateralis of healthy humans before, immediately after, and 1 h after fatiguing resistance exercise of the lower limbs. Biopsies were analyzed for nuclear NF-κB DNA binding activity by using electrophoretic mobility shift assay. NF-κB activity, measured immediately after exercise, was less than preexercise activity; after 1-h recovery, activity returned to preexercise levels. In follow-up studies in adult mice, basal NF-κB activity varied among individual muscles. NF-κB activity in diaphragm fiber bundles was decreased after a 10-min bout of fatiguing tetanic contractions in. vitro. NF-κB activity in soleus was increased by 12 days of unloading by hindlimb suspension; this increase was reversed by 10 min of fatiguing exercise. These data provide no support for our original hypothesis. Instead, acute fatiguing exercise appears to decrease NF-κB activity in muscle under a variety of conditions.

Original languageEnglish (US)
Pages (from-to)1740-1745
Number of pages6
JournalJournal of Applied Physiology
Volume97
Issue number5
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Skeletal Muscle
Muscles
DNA
Hindlimb Suspension
Biopsy
Muscle Proteins
Quadriceps Muscle
Electrophoretic Mobility Shift Assay
Muscle Contraction
Diaphragm
Lower Extremity
Exercise

Keywords

  • Muscle contraction
  • Oxidative stress
  • Signal transduction
  • Transcription factor

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Durham, W. J., Li, Y. P., Gerken, E., Farid, M., Arbogast, S., Wolfe, R. R., & Reid, M. B. (2004). Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei. Journal of Applied Physiology, 97(5), 1740-1745. https://doi.org/10.1152/japplphysiol.00088.2004

Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei. / Durham, William J.; Li, Yi Ping; Gerken, Eric; Farid, Mehran; Arbogast, Sandrine; Wolfe, Robert R.; Reid, Michael B.

In: Journal of Applied Physiology, Vol. 97, No. 5, 11.2004, p. 1740-1745.

Research output: Contribution to journalArticle

Durham, WJ, Li, YP, Gerken, E, Farid, M, Arbogast, S, Wolfe, RR & Reid, MB 2004, 'Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei', Journal of Applied Physiology, vol. 97, no. 5, pp. 1740-1745. https://doi.org/10.1152/japplphysiol.00088.2004
Durham, William J. ; Li, Yi Ping ; Gerken, Eric ; Farid, Mehran ; Arbogast, Sandrine ; Wolfe, Robert R. ; Reid, Michael B. / Fatiguing exercise reduces DNA binding activity of NF-κB in skeletal muscle nuclei. In: Journal of Applied Physiology. 2004 ; Vol. 97, No. 5. pp. 1740-1745.
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