Fatty acid conjugates of chlorinated phenols and their high-performance liquid chromatographic analysis

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Abstract

Fatty acid (C16 and C18) conjugates of pentachlorophenol and four other commonly used chlorinated phenols (2,4-di-, 2,4,5- and 2,4,6-tri-, and 2,3,4,6-tetra-chlorophenols) were systhesized and their structures were established by proton nuclear magnetic resonance spectroscopy and chenmical ionization mass spctrometry. The high-performance liquid chromatographic (HPLC) separation was achieved on reversed-phase (C-18) column using methanol-water (39:1, v/v) at a flow-rate of 1 ml/min. Palmitoyl- and oleoyl-2,4-dichlorophenols (19.6 min), palmitoyl- and oleoyl-2,4,5-trichlorophenols and stearoyl-2,4-dichlorophenols (28.0 min), oleoyl-2,4,6-trichlorophenol and linoleoyl-2,3,4,6-tetrachlorophenol (30.8 min), palmitoyl- and oleoyl-2,3,4,6-tetrachlorophenols and stearoyl-2,4,5-trichlorophenol (43.2 min) and palmitoyl- and oleoyl-petachlorophenols (72.0 min) were co-eluted under the HPLC conditions described above. Therefore, reversed-phase HPLC separation of a mixture of the five chlorinated phenol conjugates of each fatty acid was achieved.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalJournal of Chromatography A
Volume537
Issue numberC
DOIs
StatePublished - 1991

Fingerprint

Phenols
High performance liquid chromatography
Fatty Acids
High Pressure Liquid Chromatography
Chlorophenols
Pentachlorophenol
Liquids
Phenol
Methanol
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy
Ionization
Water
Flow rate
Nuclear magnetic resonance
2,4,5-trichlorophenol
2,3,4,6-tetrachlorophenol
2,4-dichlorophenol
Proton Magnetic Resonance Spectroscopy
2,4,6-trichlorophenol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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title = "Fatty acid conjugates of chlorinated phenols and their high-performance liquid chromatographic analysis",
abstract = "Fatty acid (C16 and C18) conjugates of pentachlorophenol and four other commonly used chlorinated phenols (2,4-di-, 2,4,5- and 2,4,6-tri-, and 2,3,4,6-tetra-chlorophenols) were systhesized and their structures were established by proton nuclear magnetic resonance spectroscopy and chenmical ionization mass spctrometry. The high-performance liquid chromatographic (HPLC) separation was achieved on reversed-phase (C-18) column using methanol-water (39:1, v/v) at a flow-rate of 1 ml/min. Palmitoyl- and oleoyl-2,4-dichlorophenols (19.6 min), palmitoyl- and oleoyl-2,4,5-trichlorophenols and stearoyl-2,4-dichlorophenols (28.0 min), oleoyl-2,4,6-trichlorophenol and linoleoyl-2,3,4,6-tetrachlorophenol (30.8 min), palmitoyl- and oleoyl-2,3,4,6-tetrachlorophenols and stearoyl-2,4,5-trichlorophenol (43.2 min) and palmitoyl- and oleoyl-petachlorophenols (72.0 min) were co-eluted under the HPLC conditions described above. Therefore, reversed-phase HPLC separation of a mixture of the five chlorinated phenol conjugates of each fatty acid was achieved.",
author = "Bhupendra Kaphalia",
year = "1991",
doi = "10.1016/S0021-9673(01)88888-9",
language = "English (US)",
volume = "537",
pages = "85--92",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "C",

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TY - JOUR

T1 - Fatty acid conjugates of chlorinated phenols and their high-performance liquid chromatographic analysis

AU - Kaphalia, Bhupendra

PY - 1991

Y1 - 1991

N2 - Fatty acid (C16 and C18) conjugates of pentachlorophenol and four other commonly used chlorinated phenols (2,4-di-, 2,4,5- and 2,4,6-tri-, and 2,3,4,6-tetra-chlorophenols) were systhesized and their structures were established by proton nuclear magnetic resonance spectroscopy and chenmical ionization mass spctrometry. The high-performance liquid chromatographic (HPLC) separation was achieved on reversed-phase (C-18) column using methanol-water (39:1, v/v) at a flow-rate of 1 ml/min. Palmitoyl- and oleoyl-2,4-dichlorophenols (19.6 min), palmitoyl- and oleoyl-2,4,5-trichlorophenols and stearoyl-2,4-dichlorophenols (28.0 min), oleoyl-2,4,6-trichlorophenol and linoleoyl-2,3,4,6-tetrachlorophenol (30.8 min), palmitoyl- and oleoyl-2,3,4,6-tetrachlorophenols and stearoyl-2,4,5-trichlorophenol (43.2 min) and palmitoyl- and oleoyl-petachlorophenols (72.0 min) were co-eluted under the HPLC conditions described above. Therefore, reversed-phase HPLC separation of a mixture of the five chlorinated phenol conjugates of each fatty acid was achieved.

AB - Fatty acid (C16 and C18) conjugates of pentachlorophenol and four other commonly used chlorinated phenols (2,4-di-, 2,4,5- and 2,4,6-tri-, and 2,3,4,6-tetra-chlorophenols) were systhesized and their structures were established by proton nuclear magnetic resonance spectroscopy and chenmical ionization mass spctrometry. The high-performance liquid chromatographic (HPLC) separation was achieved on reversed-phase (C-18) column using methanol-water (39:1, v/v) at a flow-rate of 1 ml/min. Palmitoyl- and oleoyl-2,4-dichlorophenols (19.6 min), palmitoyl- and oleoyl-2,4,5-trichlorophenols and stearoyl-2,4-dichlorophenols (28.0 min), oleoyl-2,4,6-trichlorophenol and linoleoyl-2,3,4,6-tetrachlorophenol (30.8 min), palmitoyl- and oleoyl-2,3,4,6-tetrachlorophenols and stearoyl-2,4,5-trichlorophenol (43.2 min) and palmitoyl- and oleoyl-petachlorophenols (72.0 min) were co-eluted under the HPLC conditions described above. Therefore, reversed-phase HPLC separation of a mixture of the five chlorinated phenol conjugates of each fatty acid was achieved.

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