Fatty acid ethyl ester synthase, an enzyme for nonoxidative ethanol metabolism, is present in serum after liver and pancreatic injury

Samir Aleryani, Ayman Kabakibi, Joanne Cluette-Brown, Michael Laposata

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Fatty acid ethyl esters (FAEE), esterification products of ethanol and fatty acids, have been implicated as mediators of ethanol-induced organ damage. Because cytosolic enzymes such as aspartate aminotransferase, lipase, and amylase appear in the blood after liver or pancreatic damage, we hypothesized that FAEE synthase, which is both cytosolic and membrane bound, is also released into the blond of patients with liver or pancreatic disease. We used a method involving thin-layer chromatography coupled with gas chromatography-mass spectrometry to reliably identify and quantify FAEE. In this study, we demonstrated that patients with liver or pancreatic disease release FAEE synthase into their plasma in amounts proportional to the amount of aspartate aminotransferase (r = 0.78), anlylase (r = 0.65), and lipase (r = 0.63). These data indicate that liver and pancreatic damage results in release of FAEE synthase into the blood. The presence of FAEE synthase in plasma permits nonoxidative ethanol metabolism in the plasma.

Original languageEnglish (US)
Pages (from-to)24-27
Number of pages4
JournalClinical Chemistry
Volume42
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Metabolism
Liver
Ethanol
Pancreatic Diseases
Fatty Acids
Wounds and Injuries
Enzymes
Aspartate Aminotransferases
Serum
Lipase
Plasmas
Liver Diseases
Esters
Blood
Thin layer chromatography
Esterification
Amylases
Thin Layer Chromatography
Gas chromatography
Gas Chromatography-Mass Spectrometry

Keywords

  • chromatography, thin-layer
  • enzyme activity
  • fatty acids
  • gas chromatography-mass spectrometry
  • lipids

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Fatty acid ethyl ester synthase, an enzyme for nonoxidative ethanol metabolism, is present in serum after liver and pancreatic injury. / Aleryani, Samir; Kabakibi, Ayman; Cluette-Brown, Joanne; Laposata, Michael.

In: Clinical Chemistry, Vol. 42, No. 1, 1996, p. 24-27.

Research output: Contribution to journalArticle

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