Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis

Saam Motamedi, Tuya Shilagard, Luke Koong, Gracie Vargas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Gold nanorods (GNRs) combined with two-photon microscopy were explored for potential application in imaging of oral carcinogenesis. GNRs have been shown to be effective contrast agents for two photon luminescence in that excitation laser powers required for imaging are low compared to traditional fluorophores. Imaging of cells, ex vivo tissues, and in vivo oral mucosa labeled with GNRs was performed to evaluate potential advantages of these agents in molecular imaging of epithelial carcinogenesis. Powers required to elicit a two-photon luminescence signal from GNRs were determined for cells as well as normal and malignant transformed lesions, 24 hours following injection of GNRs in a hamster model for oral cancer. The strength of the detected emission as the function of the average incident laser power was measured in tissues with and without GNRs to compare the sensitivity of GNRs against tissue autofluorescence. Finally, in vivo imaging was performed immediately following GNR injection to establish the ability to image microvasculature at low incident powers. The pilot study demonstrated uptake of GNRs by cells and in tissues yielding bright fluorescence signals using significantly lower incident powers than those needed to excite tissue autofluorescence. The in vivo imaging aspect of the study demonstrated the localization of GNRs within the microvasculature of the oral cancer model. These preliminary studies demonstrated the ability of GNRs to function as photostable, high contrast imaging agents and suggest that GNRs and multi-photon imaging have great potential for applications in the field of molecular imaging and early detection of cancer.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7576
DOIs
StatePublished - 2010
EventReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II - San Francisco, CA, United States
Duration: Jan 25 2010Jan 27 2010

Other

OtherReporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II
CountryUnited States
CitySan Francisco, CA
Period1/25/101/27/10

Fingerprint

Nanotubes
Nanorods
Photons
Gold
nanorods
Microscopy
Microscopic examination
Carcinogenesis
gold
microscopy
photons
Imaging techniques
Tissue
Molecular imaging
Molecular Imaging
Mouth Neoplasms
cancer
Microvessels
Luminescence
Contrast Media

Keywords

  • Cancer
  • Gold nanorods
  • Imaging
  • Luminescence
  • Two photon microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Motamedi, S., Shilagard, T., Koong, L., & Vargas, G. (2010). Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7576). [75760Z] https://doi.org/10.1117/12.843036

Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis. / Motamedi, Saam; Shilagard, Tuya; Koong, Luke; Vargas, Gracie.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7576 2010. 75760Z.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Motamedi, S, Shilagard, T, Koong, L & Vargas, G 2010, Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7576, 75760Z, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, San Francisco, CA, United States, 1/25/10. https://doi.org/10.1117/12.843036
Motamedi S, Shilagard T, Koong L, Vargas G. Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7576. 2010. 75760Z https://doi.org/10.1117/12.843036
Motamedi, Saam ; Shilagard, Tuya ; Koong, Luke ; Vargas, Gracie. / Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7576 2010.
@inproceedings{315a80fc110645edaf501548bf0ee205,
title = "Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis",
abstract = "Gold nanorods (GNRs) combined with two-photon microscopy were explored for potential application in imaging of oral carcinogenesis. GNRs have been shown to be effective contrast agents for two photon luminescence in that excitation laser powers required for imaging are low compared to traditional fluorophores. Imaging of cells, ex vivo tissues, and in vivo oral mucosa labeled with GNRs was performed to evaluate potential advantages of these agents in molecular imaging of epithelial carcinogenesis. Powers required to elicit a two-photon luminescence signal from GNRs were determined for cells as well as normal and malignant transformed lesions, 24 hours following injection of GNRs in a hamster model for oral cancer. The strength of the detected emission as the function of the average incident laser power was measured in tissues with and without GNRs to compare the sensitivity of GNRs against tissue autofluorescence. Finally, in vivo imaging was performed immediately following GNR injection to establish the ability to image microvasculature at low incident powers. The pilot study demonstrated uptake of GNRs by cells and in tissues yielding bright fluorescence signals using significantly lower incident powers than those needed to excite tissue autofluorescence. The in vivo imaging aspect of the study demonstrated the localization of GNRs within the microvasculature of the oral cancer model. These preliminary studies demonstrated the ability of GNRs to function as photostable, high contrast imaging agents and suggest that GNRs and multi-photon imaging have great potential for applications in the field of molecular imaging and early detection of cancer.",
keywords = "Cancer, Gold nanorods, Imaging, Luminescence, Two photon microscopy",
author = "Saam Motamedi and Tuya Shilagard and Luke Koong and Gracie Vargas",
year = "2010",
doi = "10.1117/12.843036",
language = "English (US)",
isbn = "9780819479723",
volume = "7576",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - Feasibility of using gold nanorods for optical contrast in two photon microscopy of oral carcinogenesis

AU - Motamedi, Saam

AU - Shilagard, Tuya

AU - Koong, Luke

AU - Vargas, Gracie

PY - 2010

Y1 - 2010

N2 - Gold nanorods (GNRs) combined with two-photon microscopy were explored for potential application in imaging of oral carcinogenesis. GNRs have been shown to be effective contrast agents for two photon luminescence in that excitation laser powers required for imaging are low compared to traditional fluorophores. Imaging of cells, ex vivo tissues, and in vivo oral mucosa labeled with GNRs was performed to evaluate potential advantages of these agents in molecular imaging of epithelial carcinogenesis. Powers required to elicit a two-photon luminescence signal from GNRs were determined for cells as well as normal and malignant transformed lesions, 24 hours following injection of GNRs in a hamster model for oral cancer. The strength of the detected emission as the function of the average incident laser power was measured in tissues with and without GNRs to compare the sensitivity of GNRs against tissue autofluorescence. Finally, in vivo imaging was performed immediately following GNR injection to establish the ability to image microvasculature at low incident powers. The pilot study demonstrated uptake of GNRs by cells and in tissues yielding bright fluorescence signals using significantly lower incident powers than those needed to excite tissue autofluorescence. The in vivo imaging aspect of the study demonstrated the localization of GNRs within the microvasculature of the oral cancer model. These preliminary studies demonstrated the ability of GNRs to function as photostable, high contrast imaging agents and suggest that GNRs and multi-photon imaging have great potential for applications in the field of molecular imaging and early detection of cancer.

AB - Gold nanorods (GNRs) combined with two-photon microscopy were explored for potential application in imaging of oral carcinogenesis. GNRs have been shown to be effective contrast agents for two photon luminescence in that excitation laser powers required for imaging are low compared to traditional fluorophores. Imaging of cells, ex vivo tissues, and in vivo oral mucosa labeled with GNRs was performed to evaluate potential advantages of these agents in molecular imaging of epithelial carcinogenesis. Powers required to elicit a two-photon luminescence signal from GNRs were determined for cells as well as normal and malignant transformed lesions, 24 hours following injection of GNRs in a hamster model for oral cancer. The strength of the detected emission as the function of the average incident laser power was measured in tissues with and without GNRs to compare the sensitivity of GNRs against tissue autofluorescence. Finally, in vivo imaging was performed immediately following GNR injection to establish the ability to image microvasculature at low incident powers. The pilot study demonstrated uptake of GNRs by cells and in tissues yielding bright fluorescence signals using significantly lower incident powers than those needed to excite tissue autofluorescence. The in vivo imaging aspect of the study demonstrated the localization of GNRs within the microvasculature of the oral cancer model. These preliminary studies demonstrated the ability of GNRs to function as photostable, high contrast imaging agents and suggest that GNRs and multi-photon imaging have great potential for applications in the field of molecular imaging and early detection of cancer.

KW - Cancer

KW - Gold nanorods

KW - Imaging

KW - Luminescence

KW - Two photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=77951774175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951774175&partnerID=8YFLogxK

U2 - 10.1117/12.843036

DO - 10.1117/12.843036

M3 - Conference contribution

SN - 9780819479723

VL - 7576

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -