Fentanyl enhances HIV infection in vitro

Jie Yan; Dong hang Nie; Cheng si Bai; Abdul Rehman; An Yang; Xiao Li Mou; Yu qing Zhang; Ying qi Xu; Qing qing Xiang; Yu ting Ren; Jia le Xu; Mei rong Wang; Yong Feng; Xiao ping Chen; Yong Xiong; Hai tao Hu; Hai rong Xiong; Wei Hou

doi:10.1016/j.virol.2022.10.004

Fentanyl enhances HIV infection in vitro

Jie Yan, Dong hang Nie, Cheng si Bai, Abdul Rehman, An Yang, Xiao Li Mou, Yu qing Zhang, Ying qi Xu, Qing qing Xiang, Yu ting Ren, Jia le Xu, Mei rong Wang, Yong Feng, Xiao ping Chen, Yong Xiong, Hai tao Hu, Hai rong Xiong, Wei Hou

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Acquired immunodeficiency syndrome (AIDS) caused by Human immunodeficiency virus type 1 (HIV-1) has a high tendency among illicit drug abusers. Recently, it is reported that abuse of fentanyl, a potent synthetic μ receptor–stimulating opioid, is an independent risk factor for HIV-1 infection. However, the mechanism of action in augmenting HIV-1 infection still remains elusive. In this study, we found that fentanyl enhanced infection of HIV-1 in MT2 cells, primary macrophages and Jurkat C11 cells. Fentanyl up-regulated CXCR4 and CCR5 receptor expression, which facilitated the entry of virion into host cells. In addition, it down-regulated interferon-β (IFN-β) and interferon-stimulated genes (APOBEC3F, APOBEC3G and MxB) expression in MT2 cells. Our findings identify an essential role of fentanyl in the positive regulation of HIV-1 infection via the upregulation of co-receptors (CXCR4/CCR5) and downregulation of IFN-β and ISGs, and it may have an important role in HIV-1 immunopathogenesis.

Original language	English (US)
Pages (from-to)	43-50
Number of pages	8
Journal	Virology
Volume	577
DOIs	https://doi.org/10.1016/j.virol.2022.10.004
State	Published - Dec 2022

Keywords

Acquired immunodeficiency syndrome
Fentanyl
Human immunodeficiency virus
Opioids
Reactivation

ASJC Scopus subject areas

Virology

Access to Document

10.1016/j.virol.2022.10.004

Cite this

@article{6d21c38188c8492fa905d10f2e89c164,

title = "Fentanyl enhances HIV infection in vitro",

abstract = "Acquired immunodeficiency syndrome (AIDS) caused by Human immunodeficiency virus type 1 (HIV-1) has a high tendency among illicit drug abusers. Recently, it is reported that abuse of fentanyl, a potent synthetic μ receptor–stimulating opioid, is an independent risk factor for HIV-1 infection. However, the mechanism of action in augmenting HIV-1 infection still remains elusive. In this study, we found that fentanyl enhanced infection of HIV-1 in MT2 cells, primary macrophages and Jurkat C11 cells. Fentanyl up-regulated CXCR4 and CCR5 receptor expression, which facilitated the entry of virion into host cells. In addition, it down-regulated interferon-β (IFN-β) and interferon-stimulated genes (APOBEC3F, APOBEC3G and MxB) expression in MT2 cells. Our findings identify an essential role of fentanyl in the positive regulation of HIV-1 infection via the upregulation of co-receptors (CXCR4/CCR5) and downregulation of IFN-β and ISGs, and it may have an important role in HIV-1 immunopathogenesis.",

keywords = "Acquired immunodeficiency syndrome, Fentanyl, Human immunodeficiency virus, Opioids, Reactivation",

author = "Jie Yan and Nie, {Dong hang} and Bai, {Cheng si} and Abdul Rehman and An Yang and Mou, {Xiao Li} and Zhang, {Yu qing} and Xu, {Ying qi} and Xiang, {Qing qing} and Ren, {Yu ting} and Xu, {Jia le} and Wang, {Mei rong} and Yong Feng and Chen, {Xiao ping} and Yong Xiong and Hu, {Hai tao} and Xiong, {Hai rong} and Wei Hou",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

year = "2022",

month = dec,

doi = "10.1016/j.virol.2022.10.004",

language = "English (US)",

volume = "577",

pages = "43--50",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Fentanyl enhances HIV infection in vitro

AU - Yan, Jie

AU - Nie, Dong hang

AU - Bai, Cheng si

AU - Rehman, Abdul

AU - Yang, An

AU - Mou, Xiao Li

AU - Zhang, Yu qing

AU - Xu, Ying qi

AU - Xiang, Qing qing

AU - Ren, Yu ting

AU - Xu, Jia le

AU - Wang, Mei rong

AU - Feng, Yong

AU - Chen, Xiao ping

AU - Xiong, Yong

AU - Hu, Hai tao

AU - Xiong, Hai rong

AU - Hou, Wei

PY - 2022/12

Y1 - 2022/12

N2 - Acquired immunodeficiency syndrome (AIDS) caused by Human immunodeficiency virus type 1 (HIV-1) has a high tendency among illicit drug abusers. Recently, it is reported that abuse of fentanyl, a potent synthetic μ receptor–stimulating opioid, is an independent risk factor for HIV-1 infection. However, the mechanism of action in augmenting HIV-1 infection still remains elusive. In this study, we found that fentanyl enhanced infection of HIV-1 in MT2 cells, primary macrophages and Jurkat C11 cells. Fentanyl up-regulated CXCR4 and CCR5 receptor expression, which facilitated the entry of virion into host cells. In addition, it down-regulated interferon-β (IFN-β) and interferon-stimulated genes (APOBEC3F, APOBEC3G and MxB) expression in MT2 cells. Our findings identify an essential role of fentanyl in the positive regulation of HIV-1 infection via the upregulation of co-receptors (CXCR4/CCR5) and downregulation of IFN-β and ISGs, and it may have an important role in HIV-1 immunopathogenesis.

AB - Acquired immunodeficiency syndrome (AIDS) caused by Human immunodeficiency virus type 1 (HIV-1) has a high tendency among illicit drug abusers. Recently, it is reported that abuse of fentanyl, a potent synthetic μ receptor–stimulating opioid, is an independent risk factor for HIV-1 infection. However, the mechanism of action in augmenting HIV-1 infection still remains elusive. In this study, we found that fentanyl enhanced infection of HIV-1 in MT2 cells, primary macrophages and Jurkat C11 cells. Fentanyl up-regulated CXCR4 and CCR5 receptor expression, which facilitated the entry of virion into host cells. In addition, it down-regulated interferon-β (IFN-β) and interferon-stimulated genes (APOBEC3F, APOBEC3G and MxB) expression in MT2 cells. Our findings identify an essential role of fentanyl in the positive regulation of HIV-1 infection via the upregulation of co-receptors (CXCR4/CCR5) and downregulation of IFN-β and ISGs, and it may have an important role in HIV-1 immunopathogenesis.

KW - Acquired immunodeficiency syndrome

KW - Fentanyl

KW - Human immunodeficiency virus

KW - Opioids

KW - Reactivation

UR - http://www.scopus.com/inward/record.url?scp=85140245339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85140245339&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2022.10.004

DO - 10.1016/j.virol.2022.10.004

M3 - Article

C2 - 36279602

AN - SCOPUS:85140245339

SN - 0042-6822

VL - 577

SP - 43

EP - 50

JO - Virology

JF - Virology

ER -

Fentanyl enhances HIV infection in vitro

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this