Ferritin and desferrioxamine attenuate xanthine oxidase-dependent leak in isolated perfused rat lungs

Brooks M. Hybertson, Kevin G. Connelly, Raquel T. Buser, John E. Repine

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Iron, through its participation in reactions that generate reactive oxygen species, may contribute to the oxidative lung injury observed in patients with acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS). A number of investigators have shown that the endogenous iron storage protein ferritin increases in the blood of patients with and at-risk for ALI and ARDS, but the significance of these increases are not known. In the present investigation, we measured lung tissue levels of thiobarbituric acid reactive substances (TBARS) and lung leak in isolated rat lungs perfused with xanthine oxidase (XO) and purine, an enzymatic system which generates reactive oxygen species. We found that adding ferritin (100 ng/mL) or desferrioxamine (DFO, 10 mM), an iron chelator, to the vascular perfusate solution decreased oxidant-induced leak in isolated rat lungs perfused with XO and purine. Addition of ferritin or DFO also decreased TBARS in isolated rat lungs perfused with XO and purine; neither ferritin nor DFO, however, decreased XO activity in vitro. Our results suggest that oxidative lung leak may be altered by the availability of reactive iron and that ferritin may contribute to protection against oxidative lung injury.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalInflammation
Volume26
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Deferoxamine
Xanthine Oxidase
Ferritins
Lung
Iron
Thiobarbituric Acid Reactive Substances
Acute Lung Injury
Adult Respiratory Distress Syndrome
Lung Injury
Reactive Oxygen Species
Chelating Agents
Oxidants
Blood Vessels
Research Personnel
Proteins

Keywords

  • Acute lung injury
  • Antioxidants
  • Ferritin
  • Iron
  • Oxidants

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

Cite this

Ferritin and desferrioxamine attenuate xanthine oxidase-dependent leak in isolated perfused rat lungs. / Hybertson, Brooks M.; Connelly, Kevin G.; Buser, Raquel T.; Repine, John E.

In: Inflammation, Vol. 26, No. 4, 2002, p. 153-159.

Research output: Contribution to journalArticle

Hybertson, Brooks M. ; Connelly, Kevin G. ; Buser, Raquel T. ; Repine, John E. / Ferritin and desferrioxamine attenuate xanthine oxidase-dependent leak in isolated perfused rat lungs. In: Inflammation. 2002 ; Vol. 26, No. 4. pp. 153-159.
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