Fetal and neoplastic expression of the neurotensin gene in the human colon

B. Mark Evers, Zhichao Zhou, Vicky Dohlen, Srinivasan Rajaraman, James C. Thompson, Courtney Townsend

Research output: Contribution to journalArticle

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Abstract

Objective: The authors identified various colon cancers that express the gene for the gut peptide neurotensin (NT/N). In addition, the authors sought to delineate the temporal pattern of NT/N gene expression in the human fetal colon. Summary Background Data: Expression of NT/N is localized to the mucosa of the adult small bowel but also has been identified in the fetal colon, which resembles the small bowel until the end of the second trimester. Ectopic NT/N expression has been shown in certain types of colon cancer, suggesting a reversion to a fetal phenotype. Methods: Sensitive ribonuclease protection assays were used to determine NT/N expression in colon cancers and adjacent normal mucosa as well as color cancers established as tumor xenografts and fetal colon samples. Results: NT/N gene expression was shown in 4 of 12 (25%) human colon cancer xenografts and in 11 of 40 (28%) freshly resected colon adenocarcinomas; NT/N gene expression was not expressed in any of the samples of normal colonic mucosa adjacent to the tumors. The NT/N gene was expressed maximally in the fetal colon between 16 and 18 weeks' gestation; NT/N expression was decreased between 19 and 22 weeks and was not apparent in either the 24-week fetal colon or the adult samples. Conclusions: The NT/N gene expression is expressed transiently in the fetal colon during a development stage that is characterized by morphologic similarity to the small bowel. In addition, NT/N is reexpressed in approximately one fourth of the human colon cancers, indicating that neoplastic transformation leads to reversion to a fetal phenotype in certain types of colon cancer. The NT/N gene will provide a useful model to further define the complex differentiation pathways in the normal gut as well as the process of fetal 'dedifferentiation' in certain types of colon cancer.

Original languageEnglish (US)
Pages (from-to)464-471
Number of pages8
JournalAnnals of Surgery
Volume223
Issue number5
DOIs
StatePublished - 1996

Fingerprint

Neurotensin
Colon
Gene Expression
Colonic Neoplasms
Mucous Membrane
Heterografts
Phenotype
Neoplasms
Neoplasm Genes
Second Pregnancy Trimester
Ribonucleases
Genes
Adenocarcinoma
Color

ASJC Scopus subject areas

  • Surgery

Cite this

Fetal and neoplastic expression of the neurotensin gene in the human colon. / Evers, B. Mark; Zhou, Zhichao; Dohlen, Vicky; Rajaraman, Srinivasan; Thompson, James C.; Townsend, Courtney.

In: Annals of Surgery, Vol. 223, No. 5, 1996, p. 464-471.

Research output: Contribution to journalArticle

Evers, B. Mark ; Zhou, Zhichao ; Dohlen, Vicky ; Rajaraman, Srinivasan ; Thompson, James C. ; Townsend, Courtney. / Fetal and neoplastic expression of the neurotensin gene in the human colon. In: Annals of Surgery. 1996 ; Vol. 223, No. 5. pp. 464-471.
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abstract = "Objective: The authors identified various colon cancers that express the gene for the gut peptide neurotensin (NT/N). In addition, the authors sought to delineate the temporal pattern of NT/N gene expression in the human fetal colon. Summary Background Data: Expression of NT/N is localized to the mucosa of the adult small bowel but also has been identified in the fetal colon, which resembles the small bowel until the end of the second trimester. Ectopic NT/N expression has been shown in certain types of colon cancer, suggesting a reversion to a fetal phenotype. Methods: Sensitive ribonuclease protection assays were used to determine NT/N expression in colon cancers and adjacent normal mucosa as well as color cancers established as tumor xenografts and fetal colon samples. Results: NT/N gene expression was shown in 4 of 12 (25{\%}) human colon cancer xenografts and in 11 of 40 (28{\%}) freshly resected colon adenocarcinomas; NT/N gene expression was not expressed in any of the samples of normal colonic mucosa adjacent to the tumors. The NT/N gene was expressed maximally in the fetal colon between 16 and 18 weeks' gestation; NT/N expression was decreased between 19 and 22 weeks and was not apparent in either the 24-week fetal colon or the adult samples. Conclusions: The NT/N gene expression is expressed transiently in the fetal colon during a development stage that is characterized by morphologic similarity to the small bowel. In addition, NT/N is reexpressed in approximately one fourth of the human colon cancers, indicating that neoplastic transformation leads to reversion to a fetal phenotype in certain types of colon cancer. The NT/N gene will provide a useful model to further define the complex differentiation pathways in the normal gut as well as the process of fetal 'dedifferentiation' in certain types of colon cancer.",
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AU - Townsend, Courtney

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N2 - Objective: The authors identified various colon cancers that express the gene for the gut peptide neurotensin (NT/N). In addition, the authors sought to delineate the temporal pattern of NT/N gene expression in the human fetal colon. Summary Background Data: Expression of NT/N is localized to the mucosa of the adult small bowel but also has been identified in the fetal colon, which resembles the small bowel until the end of the second trimester. Ectopic NT/N expression has been shown in certain types of colon cancer, suggesting a reversion to a fetal phenotype. Methods: Sensitive ribonuclease protection assays were used to determine NT/N expression in colon cancers and adjacent normal mucosa as well as color cancers established as tumor xenografts and fetal colon samples. Results: NT/N gene expression was shown in 4 of 12 (25%) human colon cancer xenografts and in 11 of 40 (28%) freshly resected colon adenocarcinomas; NT/N gene expression was not expressed in any of the samples of normal colonic mucosa adjacent to the tumors. The NT/N gene was expressed maximally in the fetal colon between 16 and 18 weeks' gestation; NT/N expression was decreased between 19 and 22 weeks and was not apparent in either the 24-week fetal colon or the adult samples. Conclusions: The NT/N gene expression is expressed transiently in the fetal colon during a development stage that is characterized by morphologic similarity to the small bowel. In addition, NT/N is reexpressed in approximately one fourth of the human colon cancers, indicating that neoplastic transformation leads to reversion to a fetal phenotype in certain types of colon cancer. The NT/N gene will provide a useful model to further define the complex differentiation pathways in the normal gut as well as the process of fetal 'dedifferentiation' in certain types of colon cancer.

AB - Objective: The authors identified various colon cancers that express the gene for the gut peptide neurotensin (NT/N). In addition, the authors sought to delineate the temporal pattern of NT/N gene expression in the human fetal colon. Summary Background Data: Expression of NT/N is localized to the mucosa of the adult small bowel but also has been identified in the fetal colon, which resembles the small bowel until the end of the second trimester. Ectopic NT/N expression has been shown in certain types of colon cancer, suggesting a reversion to a fetal phenotype. Methods: Sensitive ribonuclease protection assays were used to determine NT/N expression in colon cancers and adjacent normal mucosa as well as color cancers established as tumor xenografts and fetal colon samples. Results: NT/N gene expression was shown in 4 of 12 (25%) human colon cancer xenografts and in 11 of 40 (28%) freshly resected colon adenocarcinomas; NT/N gene expression was not expressed in any of the samples of normal colonic mucosa adjacent to the tumors. The NT/N gene was expressed maximally in the fetal colon between 16 and 18 weeks' gestation; NT/N expression was decreased between 19 and 22 weeks and was not apparent in either the 24-week fetal colon or the adult samples. Conclusions: The NT/N gene expression is expressed transiently in the fetal colon during a development stage that is characterized by morphologic similarity to the small bowel. In addition, NT/N is reexpressed in approximately one fourth of the human colon cancers, indicating that neoplastic transformation leads to reversion to a fetal phenotype in certain types of colon cancer. The NT/N gene will provide a useful model to further define the complex differentiation pathways in the normal gut as well as the process of fetal 'dedifferentiation' in certain types of colon cancer.

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