Fetal exposure to bisphenol A as a risk factor for the development of childhood asthma

An animal model study

Yoichi Nakajima, Randall M. Goldblum, Terumi Midoro-Horiuti

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background: The prevalence of asthma in industrialized countries has been increasing dramatically and asthma is now the most common chronic disease of children in the United States. The rapidity of the increase strongly suggests that changes in environmental exposures are the likely cause of this epidemic. Further, the early onset of allergic manifestations suggests that these exposures may act on the prenatal development of the immune system. We have focused on the potential effects of bisphenol A (BPA), a chemical pollutant with one of the largest productions, on the development of childhood asthma. We have reported that perinatal BPA exposure promotes the development of allergic asthma in a mouse model. The current study was designed to identify a critical period of BPA exposure and to begin elucidating the mechanisms for this susceptibility. Methods. Female BALB/c mice received 10 micro g/ml BPA in their drinking water from one week before pregnancy until the end of the study. Some of the pups were transferred in the first 48 h of life from their BPA-loaded mother to an unexposed mother, or vice versa. Half of the pups were sensitized with a low dose of the experimental allergen ovalbumin (OVA), the rest received PBS as an unsensitized controls. On day 22, the pups were challenged by inhalations of ovalbumin or PBS followed by quantification of eosinophils in and hyperreactivity of their airways, major indicators of experimental asthma in this classical mouse model. Hepatic expression of two isoforms of UDP-glucuronosyltransferase (Ugt) was quantified by quantitative RT-PCR at various ages. Results: Pups exposed to BPA in utero and through breast milk, or in utero only, displayed an asthma phenotype in response to their "suboptimal" allergic sensitization, whereas, pups only exposed to BPA postnatally from breast milk, did not. The expression of Ugt2b1, an isoform related to BPA clearance in rats, was not detectable in mouse fetuses and newborn pups, but increased by day 5 and approached adult levels by day 25. Conclusions: Prenatal exposures that produce environmentally relevant burdens of BPA, followed by postnatal allergic sensitization and challenges, promote the development of experimental allergic asthma. Delayed expression of BPA-metabolizing enzymes may explain, at least in part, the enhanced fetal susceptibility to this common environmental contaminant.

Original languageEnglish (US)
Article number8
JournalEnvironmental Health: A Global Access Science Source
Volume11
Issue number1
DOIs
StatePublished - 2012

Fingerprint

Asthma
Animal Models
Ovalbumin
Human Milk
Protein Isoforms
Mothers
bisphenol A
Glucuronosyltransferase
Environmental Exposure
Developed Countries
Eosinophils
Drinking Water
Allergens
Inhalation
Immune System
Fetus
Chronic Disease
Newborn Infant
Phenotype
Pregnancy

Keywords

  • Animal model
  • Asthma
  • Bisphenol A
  • BPA
  • Environmental estrogen
  • Enzyme
  • Fetal exposure
  • Metabolism
  • Ovalbumin
  • Ugt2b1

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Medicine(all)

Cite this

Fetal exposure to bisphenol A as a risk factor for the development of childhood asthma : An animal model study. / Nakajima, Yoichi; Goldblum, Randall M.; Midoro-Horiuti, Terumi.

In: Environmental Health: A Global Access Science Source, Vol. 11, No. 1, 8, 2012.

Research output: Contribution to journalArticle

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