FGF-2 but not FGF-1 binds fibrin and supports prolonged endothelial cell growth

A. Sahni, O. D. Altland, C. W. Francis

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Endothelial cell viability and growth are dependent on both polypeptide growth factors, and integrin-mediated matrix interactions. We have now examined the ability of fibrin-binding and non-binding growth factors to support long-term endothelial cell growth in the presence or absence of the soluble form. Endothelial cells were cultured on a fibrin surface, with or without FGF-1 or FGF-2, and proliferation was determined by 3H-thymidine incorporation. Cells cultured on fibrin with no growth factor showed minimal proliferation up to h. In contrast, when FGF-2 was incorporated into fibrin, proliferation was increased 6.5±0.6-fold, equal to growth on a fibrin surface with FGF-2 continually present in the medium. Thymidine incorporation was similar when cells were cultured on a fibrin surface that had been incubated with FGF-2 and then the growth factor removed (8.6±0.5-fold). In contrast to results with FGF-2, a surface of fibrin exposed to FGF-1 supported minimal growth, whereas growth was comparable to either FGF-1 or FGF-2 present in the medium. Comparable results were observed when proliferation was quantitated by cell counting at times up to 48 h. Binding studies demonstrated no high-affinity interaction of FGF-1 with fibrinogen or fibrin. We conclude that FGF-2 bound to fibrin supports prolonged endothelial cell growth as well as soluble FGF-2, whereas FGF- does not bind to fibrin and can support endothelial cell growth only if continually present in soluble form. Fibrin may serve as a matrix reservoir for FGF-2 to support cell growth at sites of injury or thrombosis.

Original languageEnglish (US)
Pages (from-to)1304-1310
Number of pages7
JournalJournal of Thrombosis and Haemostasis
Volume1
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

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Keywords

  • Endothelial cells
  • FGF-1
  • FGF-2
  • Fibrin

ASJC Scopus subject areas

  • Hematology

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