FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration

Thomas Iskratsch, Cheng Han Yu, Anurag Mathur, Shuaimin Liu, Virginie Stévenin, Joseph Dwyer, James Hone, Elisabeth Ehler, Michael Sheetz

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Matrix adhesions provide critical signals for cell growth or differentiation. They form through a number of distinct steps that follow integrin binding to matrix ligands. In an early step, integrins form clusters that support actin polymerization by an unknown mechanism. This raises the question of how actin polymerization occurs at the integrin clusters. We report here that a major formin in mouse fibroblasts, FHOD1, is recruited to integrin clusters, resulting in actin assembly. Using cell-spreading assays on lipid bilayers, solid substrates, and high-resolution force-sensing pillar arrays, we find that knockdown of FHOD1 impairs spreading, coordinated application of adhesive force, and adhesion maturation. Finally, we show that targeting of FHOD1 to the integrin sites depends on the direct interaction with Src family kinases and is upstream of the activation by Rho kinase. Thus, our findings provide insights into the mechanisms of cell migration with implications for development and disease.

Original languageEnglish (US)
Pages (from-to)545-559
Number of pages15
JournalDevelopmental Cell
Volume27
Issue number5
DOIs
StatePublished - Dec 9 2013
Externally publishedYes

Fingerprint

Integrins
Cell Movement
Adhesion
Actins
Polymerization
rho-Associated Kinases
Lipid bilayers
src-Family Kinases
Lipid Bilayers
Cell growth
Fibroblasts
Adhesives
Assays
Chemical activation
Ligands
Substrates
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. / Iskratsch, Thomas; Yu, Cheng Han; Mathur, Anurag; Liu, Shuaimin; Stévenin, Virginie; Dwyer, Joseph; Hone, James; Ehler, Elisabeth; Sheetz, Michael.

In: Developmental Cell, Vol. 27, No. 5, 09.12.2013, p. 545-559.

Research output: Contribution to journalArticle

Iskratsch, T, Yu, CH, Mathur, A, Liu, S, Stévenin, V, Dwyer, J, Hone, J, Ehler, E & Sheetz, M 2013, 'FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration', Developmental Cell, vol. 27, no. 5, pp. 545-559. https://doi.org/10.1016/j.devcel.2013.11.003
Iskratsch, Thomas ; Yu, Cheng Han ; Mathur, Anurag ; Liu, Shuaimin ; Stévenin, Virginie ; Dwyer, Joseph ; Hone, James ; Ehler, Elisabeth ; Sheetz, Michael. / FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. In: Developmental Cell. 2013 ; Vol. 27, No. 5. pp. 545-559.
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