Fibre type-specific satellite cell response to aerobic training in sedentary adults

Christopher Fry, Brian Noehren, Jyothi Mula, Margo F. Ubele, Philip M. Westgate, Philip A. Kern, Charlotte A. Peterson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In the present study, we sought to determine the effect of a traditional, 12 week aerobic training protocol on skeletal muscle fibre type distribution and satellite cell content in sedentary subjects. Muscle biopsies were obtained from the vastus lateralis [n = 23 subjects (six male and 17 female); body mass index 30.7 ± 1.2 kg m-2] before and after 12 weeks of aerobic training performed on a cycle ergometer. Immunohistochemical analyses were used to quantify myosin heavy chain (MyHC) isoform expression, cross-sectional area and satellite cell and myonuclear content. Following training, a decrease in MyHC hybrid type IIa/IIx fibre frequency occurred, with a concomitant increase in pure MyHC type IIa fibres. Pretraining fibre type correlated with body mass index, and the change in fibre type following training was associated with improvements in maximal oxygen consumption. Twelve weeks of aerobic training also induced increases in mean cross-sectional area in both MyHC type I and type IIa fibres. Satellite cell content was also increased following training, specifically in MyHC type I fibres, with no change in the number of satellite cells associated with MyHC type II fibres. With the increased satellite cell content following training, an increase in myonuclear number per fibre also occurred in MyHC type I fibres. Hypertrophy of MyHC type II fibres occurred without detectable myonuclear addition, suggesting that the mechanisms underlying growth in fast and slow fibres differ. These data provide intriguing evidence for a fibre type-specific role of satellite cells in muscle adaptation following aerobic training.

Original languageEnglish (US)
Pages (from-to)2625-2635
Number of pages11
JournalJournal of Physiology
Volume592
Issue number12
DOIs
StatePublished - Jun 15 2014
Externally publishedYes

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Myosin Heavy Chains
Body Mass Index
Skeletal Muscle Fibers
Quadriceps Muscle
Oxygen Consumption
Muscle Cells
Hypertrophy
Protein Isoforms
Cell Count
Biopsy
Muscles
Growth

ASJC Scopus subject areas

  • Physiology

Cite this

Fry, C., Noehren, B., Mula, J., Ubele, M. F., Westgate, P. M., Kern, P. A., & Peterson, C. A. (2014). Fibre type-specific satellite cell response to aerobic training in sedentary adults. Journal of Physiology, 592(12), 2625-2635. https://doi.org/10.1113/jphysiol.2014.271288

Fibre type-specific satellite cell response to aerobic training in sedentary adults. / Fry, Christopher; Noehren, Brian; Mula, Jyothi; Ubele, Margo F.; Westgate, Philip M.; Kern, Philip A.; Peterson, Charlotte A.

In: Journal of Physiology, Vol. 592, No. 12, 15.06.2014, p. 2625-2635.

Research output: Contribution to journalArticle

Fry, C, Noehren, B, Mula, J, Ubele, MF, Westgate, PM, Kern, PA & Peterson, CA 2014, 'Fibre type-specific satellite cell response to aerobic training in sedentary adults', Journal of Physiology, vol. 592, no. 12, pp. 2625-2635. https://doi.org/10.1113/jphysiol.2014.271288
Fry C, Noehren B, Mula J, Ubele MF, Westgate PM, Kern PA et al. Fibre type-specific satellite cell response to aerobic training in sedentary adults. Journal of Physiology. 2014 Jun 15;592(12):2625-2635. https://doi.org/10.1113/jphysiol.2014.271288
Fry, Christopher ; Noehren, Brian ; Mula, Jyothi ; Ubele, Margo F. ; Westgate, Philip M. ; Kern, Philip A. ; Peterson, Charlotte A. / Fibre type-specific satellite cell response to aerobic training in sedentary adults. In: Journal of Physiology. 2014 ; Vol. 592, No. 12. pp. 2625-2635.
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