Fibrillarin: Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis

Fangmin Xu, TuKiet T. Lam, Mark Emmett, Christopher L. Hendrickson, Hong Li, Alan G. Marshall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The mapping of the complex binding surfaces of small nucleolar ribonucleoproteins (snoRNP), fibrillarin and Nop5, was presented. The solution-phase hydrogen/deuterium exchange (HDX) was combined with liquid chromatography (LC) microelectrospray ionization Fourier transform ion cyclotron resonance mass analysis (ESI FT-ICR MS) for the mapping. Metal affinity chromatography and dialysis were used to purify the the complex protein before the HDX was performed. A decrease in HDX rate for peptic fragment 70-80 of fibrillarin was observed when fibrillarin formed a complex with Nop5.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages299-300
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Cyclotron resonance
Electrospray ionization
Deuterium
Liquid chromatography
Hydrogen
Fourier transforms
Ions
Small Nucleolar Ribonucleoproteins
Affinity chromatography
Dialysis
Ionization
Ion exchange
Metals
fibrillarin
Proteins

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Xu, F., Lam, T. T., Emmett, M., Hendrickson, C. L., Li, H., & Marshall, A. G. (2002). Fibrillarin: Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 299-300)

Fibrillarin : Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. / Xu, Fangmin; Lam, TuKiet T.; Emmett, Mark; Hendrickson, Christopher L.; Li, Hong; Marshall, Alan G.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 299-300.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xu, F, Lam, TT, Emmett, M, Hendrickson, CL, Li, H & Marshall, AG 2002, Fibrillarin: Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 299-300, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Xu F, Lam TT, Emmett M, Hendrickson CL, Li H, Marshall AG. Fibrillarin: Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 299-300
Xu, Fangmin ; Lam, TuKiet T. ; Emmett, Mark ; Hendrickson, Christopher L. ; Li, Hong ; Marshall, Alan G. / Fibrillarin : Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 299-300
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