Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis

Fangmin Xu; Tu Kiet T. Lam; Mark R. Emmett; Christopher L. Hendrickson; Hong Li; Alan G. Marshall

Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis

Fangmin Xu, Tu Kiet T. Lam, Mark R. Emmett, Christopher L. Hendrickson, Hong Li, Alan G. Marshall

Research output: Contribution to conference › Paper › peer-review

Abstract

The mapping of the complex binding surfaces of small nucleolar ribonucleoproteins (snoRNP), fibrillarin and Nop5, was presented. The solution-phase hydrogen/deuterium exchange (HDX) was combined with liquid chromatography (LC) microelectrospray ionization Fourier transform ion cyclotron resonance mass analysis (ESI FT-ICR MS) for the mapping. Metal affinity chromatography and dialysis were used to purify the the complex protein before the HDX was performed. A decrease in HDX rate for peptic fragment 70-80 of fibrillarin was observed when fibrillarin formed a complex with Nop5.

Original language	English (US)
Pages	299-300
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Xu, F., Lam, T. K. T., Emmett, M. R., Hendrickson, C. L., Li, H., & Marshall, A. G. (2002). Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. 299-300. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis. / Xu, Fangmin; Lam, Tu Kiet T.; Emmett, Mark R. et al.
2002. 299-300 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

Xu, F, Lam, TKT, Emmett, MR, Hendrickson, CL, Li, H & Marshall, AG 2002, 'Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 299-300.

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title = "Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis",

abstract = "The mapping of the complex binding surfaces of small nucleolar ribonucleoproteins (snoRNP), fibrillarin and Nop5, was presented. The solution-phase hydrogen/deuterium exchange (HDX) was combined with liquid chromatography (LC) microelectrospray ionization Fourier transform ion cyclotron resonance mass analysis (ESI FT-ICR MS) for the mapping. Metal affinity chromatography and dialysis were used to purify the the complex protein before the HDX was performed. A decrease in HDX rate for peptic fragment 70-80 of fibrillarin was observed when fibrillarin formed a complex with Nop5.",

author = "Fangmin Xu and Lam, {Tu Kiet T.} and Emmett, {Mark R.} and Hendrickson, {Christopher L.} and Hong Li and Marshall, {Alan G.}",

year = "2002",

language = "English (US)",

pages = "299--300",

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AU - Xu, Fangmin

AU - Lam, Tu Kiet T.

AU - Emmett, Mark R.

AU - Hendrickson, Christopher L.

AU - Li, Hong

AU - Marshall, Alan G.

PY - 2002

Y1 - 2002

N2 - The mapping of the complex binding surfaces of small nucleolar ribonucleoproteins (snoRNP), fibrillarin and Nop5, was presented. The solution-phase hydrogen/deuterium exchange (HDX) was combined with liquid chromatography (LC) microelectrospray ionization Fourier transform ion cyclotron resonance mass analysis (ESI FT-ICR MS) for the mapping. Metal affinity chromatography and dialysis were used to purify the the complex protein before the HDX was performed. A decrease in HDX rate for peptic fragment 70-80 of fibrillarin was observed when fibrillarin formed a complex with Nop5.

AB - The mapping of the complex binding surfaces of small nucleolar ribonucleoproteins (snoRNP), fibrillarin and Nop5, was presented. The solution-phase hydrogen/deuterium exchange (HDX) was combined with liquid chromatography (LC) microelectrospray ionization Fourier transform ion cyclotron resonance mass analysis (ESI FT-ICR MS) for the mapping. Metal affinity chromatography and dialysis were used to purify the the complex protein before the HDX was performed. A decrease in HDX rate for peptic fragment 70-80 of fibrillarin was observed when fibrillarin formed a complex with Nop5.

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T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Fibrillarin:Nop5 complex binding surfaces mapped by H/D exchange and LC micro-electrospray ionization fourier transform ion cyclotron resonance mass analysis

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ASJC Scopus subject areas

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