Fibrinogen and fibrin protect fibroblast growth factor-2 from proteolytic degradation

Abha Sahni, Christopher A. Baker, Lee Ann Sporn, Charles W. Francis

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We have recently reported that fibrinogen and fibrin bind to fibroblast growth factor-2 (FGF-2) and potentiate its ability to stimulate proliferation of endothelial cells. In the present report, we have investigated the potential of fibrinogen and fibrin to protect FGF-2 from proteolytic degradation. FGF-2 was incubated with trypsin or chymotrypsin in the presence or absence of fibrinogen or fibrin and proteolysis of FGF-2 was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. In the absence of fibrinogen there was progressive tryptic degradation of FGF-2, but in the presence of fibrinogen, FGF-2 was completely protected from trypsin with no evidence of degradation. The degree of protection was maximum at a molar ratio of FGF-2 to fibrinogen 1:2. Fibrinogen afforded similar protection from degradation by chymotrypsin. Polymerized fibrin provided partial protection of FGF-2 from tryptic degradation, with intact FGF-2 present for up to 360 min. Fibrin provided nearly complete protection from chymotrypsin. These observations indicate that binding of FGF-2 to fibrinogen or fibrin provides protection from proteolytic degradation, and this may modulate its cell proliferative activity.

Original languageEnglish (US)
Pages (from-to)736-741
Number of pages6
JournalThrombosis and Haemostasis
Volume83
Issue number5
StatePublished - 2000
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Fibrin
Fibrinogen
Chymotrypsin
Sodium Dodecyl Sulfate
Trypsin
Proteolysis
Polyacrylamide Gel Electrophoresis
Endothelial Cells
Western Blotting

Keywords

  • FGF-2
  • Fibrinogen
  • Proteolysis
  • Trypsin

ASJC Scopus subject areas

  • Hematology

Cite this

Fibrinogen and fibrin protect fibroblast growth factor-2 from proteolytic degradation. / Sahni, Abha; Baker, Christopher A.; Sporn, Lee Ann; Francis, Charles W.

In: Thrombosis and Haemostasis, Vol. 83, No. 5, 2000, p. 736-741.

Research output: Contribution to journalArticle

Sahni, Abha ; Baker, Christopher A. ; Sporn, Lee Ann ; Francis, Charles W. / Fibrinogen and fibrin protect fibroblast growth factor-2 from proteolytic degradation. In: Thrombosis and Haemostasis. 2000 ; Vol. 83, No. 5. pp. 736-741.
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