Fibroblasts modulate intestinal secretory responses to inflammatory mediators

Helen M. Berschneider, Don W. Powell

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Cultured colonic epithelial cells and fibroblasts were used to examine the interaction between these cell types during intestinal secretion. Secretory responses of T84 colonie epithelial cells, measured as changes in the short-circuit current in modified Ussing chambers to bradykinin, serotonin, hydrogen peroxide, and histamine, were enhanced in the presence of fibroblasts, either in cocultures or when separate cultures of fibroblasts were acutely juxtaposed with the T84 cultures. This effect was abolished by pretreatment with indomethacin and the fibroblasts were found to release prostaglandin E2 in response to these inflammatory mediators. Fibroblasts may exert a paracrine regulation on the secretory response of intestinal epithelial cells via the generation and release of cyclooxygenase products in response to inflammatory mediators. These studies suggest a novel function for the intestinal fibroblastic sheath: that of amplification of the inflammatory response through mesenchymal/epithelial interaction.

Original languageEnglish (US)
Pages (from-to)484-489
Number of pages6
JournalJournal of Clinical Investigation
Volume89
Issue number2
StatePublished - 1992

Fingerprint

Fibroblasts
Epithelial Cells
Intestinal Secretions
Bradykinin
Prostaglandin-Endoperoxide Synthases
Coculture Techniques
Dinoprostone
Cell Communication
Indomethacin
Hydrogen Peroxide
Histamine
Serotonin

Keywords

  • Colonic epithelial cells
  • Fibroblasts
  • Inflammatory mediators
  • Intestinal secretion

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fibroblasts modulate intestinal secretory responses to inflammatory mediators. / Berschneider, Helen M.; Powell, Don W.

In: Journal of Clinical Investigation, Vol. 89, No. 2, 1992, p. 484-489.

Research output: Contribution to journalArticle

Berschneider, Helen M. ; Powell, Don W. / Fibroblasts modulate intestinal secretory responses to inflammatory mediators. In: Journal of Clinical Investigation. 1992 ; Vol. 89, No. 2. pp. 484-489.
@article{db628827a73a4cd3ab646c497e5b9129,
title = "Fibroblasts modulate intestinal secretory responses to inflammatory mediators",
abstract = "Cultured colonic epithelial cells and fibroblasts were used to examine the interaction between these cell types during intestinal secretion. Secretory responses of T84 colonie epithelial cells, measured as changes in the short-circuit current in modified Ussing chambers to bradykinin, serotonin, hydrogen peroxide, and histamine, were enhanced in the presence of fibroblasts, either in cocultures or when separate cultures of fibroblasts were acutely juxtaposed with the T84 cultures. This effect was abolished by pretreatment with indomethacin and the fibroblasts were found to release prostaglandin E2 in response to these inflammatory mediators. Fibroblasts may exert a paracrine regulation on the secretory response of intestinal epithelial cells via the generation and release of cyclooxygenase products in response to inflammatory mediators. These studies suggest a novel function for the intestinal fibroblastic sheath: that of amplification of the inflammatory response through mesenchymal/epithelial interaction.",
keywords = "Colonic epithelial cells, Fibroblasts, Inflammatory mediators, Intestinal secretion",
author = "Berschneider, {Helen M.} and Powell, {Don W.}",
year = "1992",
language = "English (US)",
volume = "89",
pages = "484--489",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "2",

}

TY - JOUR

T1 - Fibroblasts modulate intestinal secretory responses to inflammatory mediators

AU - Berschneider, Helen M.

AU - Powell, Don W.

PY - 1992

Y1 - 1992

N2 - Cultured colonic epithelial cells and fibroblasts were used to examine the interaction between these cell types during intestinal secretion. Secretory responses of T84 colonie epithelial cells, measured as changes in the short-circuit current in modified Ussing chambers to bradykinin, serotonin, hydrogen peroxide, and histamine, were enhanced in the presence of fibroblasts, either in cocultures or when separate cultures of fibroblasts were acutely juxtaposed with the T84 cultures. This effect was abolished by pretreatment with indomethacin and the fibroblasts were found to release prostaglandin E2 in response to these inflammatory mediators. Fibroblasts may exert a paracrine regulation on the secretory response of intestinal epithelial cells via the generation and release of cyclooxygenase products in response to inflammatory mediators. These studies suggest a novel function for the intestinal fibroblastic sheath: that of amplification of the inflammatory response through mesenchymal/epithelial interaction.

AB - Cultured colonic epithelial cells and fibroblasts were used to examine the interaction between these cell types during intestinal secretion. Secretory responses of T84 colonie epithelial cells, measured as changes in the short-circuit current in modified Ussing chambers to bradykinin, serotonin, hydrogen peroxide, and histamine, were enhanced in the presence of fibroblasts, either in cocultures or when separate cultures of fibroblasts were acutely juxtaposed with the T84 cultures. This effect was abolished by pretreatment with indomethacin and the fibroblasts were found to release prostaglandin E2 in response to these inflammatory mediators. Fibroblasts may exert a paracrine regulation on the secretory response of intestinal epithelial cells via the generation and release of cyclooxygenase products in response to inflammatory mediators. These studies suggest a novel function for the intestinal fibroblastic sheath: that of amplification of the inflammatory response through mesenchymal/epithelial interaction.

KW - Colonic epithelial cells

KW - Fibroblasts

KW - Inflammatory mediators

KW - Intestinal secretion

UR - http://www.scopus.com/inward/record.url?scp=0026594777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026594777&partnerID=8YFLogxK

M3 - Article

VL - 89

SP - 484

EP - 489

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 2

ER -