Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase η

M. Todd Washington, Robert E. Johnson, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The yeast RAD30 gene functions in error-free replication of UV-damaged DNA, and RAD30 encodes a DNA polymerase, pol η, that has the ability to efficiently and correctly replicate past a cis-syn-thymine-thymine dimer in template DNA. To better understand the role of pol ν in damage bypass, we examined its fidelity and processivity on nondamaged DNA templates. Steady- state kinetic analyses of deoxynucleotide incorporation indicate that pol η has a low fidelity, misincorporating deoxynucleotides with a frequency of about 10-2 to 10-3. Also pol η has a low processivity, incorporating only a few nucleotides before dissociating. We suggest that pol η's low fidelity reflects a flexibility in its active site rendering it more tolerant of DNA damage, while its low processivity limits its activity to reduce errors.

Original languageEnglish (US)
Pages (from-to)36835-36838
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number52
DOIs
StatePublished - Dec 24 1999

Fingerprint

DNA-Directed DNA Polymerase
Yeast
Saccharomyces cerevisiae
DNA
Pyrimidine Dimers
Thymine
DNA Damage
Catalytic Domain
Nucleotides
Yeasts
Genes
Kinetics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase η. / Washington, M. Todd; Johnson, Robert E.; Prakash, Satya; Prakash, Louise.

In: Journal of Biological Chemistry, Vol. 274, No. 52, 24.12.1999, p. 36835-36838.

Research output: Contribution to journalArticle

Washington, M. Todd ; Johnson, Robert E. ; Prakash, Satya ; Prakash, Louise. / Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase η. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 52. pp. 36835-36838.
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