Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions

Christopher D. Lynch, Nils C. Gauthier, Nicolas Biais, Andre M. Lazar, Pere Roca-Cusachs, Cheng Han Yu, Michael Sheetz

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cell motility is an essential process that depends on a coherent, cross-linked actin cytoskeleton that physically coordinates the actions of numerous structural and signaling molecules. The actin cross-linking protein, filamin (Fln), has been implicated in the support of three-dimensional cortical actin networks capable of both maintaining cellular integrity and withstanding large forces. Although numerous studies have examined cells lacking one of the multiple Fln isoforms, compensatory mechanisms can mask novel phenotypes only observable by further Fln depletion. Indeed, shRNA-mediated knockdown of FlnA in FlnB-/- mouse embryonic fibroblasts (MEFs) causes a novel endoplasmic spreading deficiency as detected by endoplasmic reticulum markers. Microtubule (MT) extension rates are also decreased but not by peripheral actin flow, because this is also decreased in the Fln-depleted system. Additionally, Fln-depleted MEFs exhibit decreased adhesion stability that appears in increased ruffling of the cell edge, reduced adhesion size, transient traction forces, and decreased stress fibers. FlnA-/- MEFs, but not FlnB-/- MEFs, also show a moderate defect in endoplasm spreading, characterized by initial extension followed by abrupt retractions and stress fiber fracture. FlnA localizes to actin linkages surrounding the endoplasm, adhesions, and stress fibers. Thus we suggest that Flns have a major role in the maintenance of actin-based mechanical linkages that enable endoplasmic spreading and MT extension as well as sustained traction forces and mature focal adhesions.

Original languageEnglish (US)
Pages (from-to)1263-1273
Number of pages11
JournalMolecular Biology of the Cell
Volume22
Issue number8
DOIs
StatePublished - Apr 15 2011
Externally publishedYes

Fingerprint

Filamins
Actins
Stress Fibers
Fibroblasts
Traction
Microtubules
Stress Fractures
Focal Adhesions
Masks
Actin Cytoskeleton
Endoplasmic Reticulum
Small Interfering RNA
Cell Movement
Protein Isoforms
Maintenance
Phenotype
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Lynch, C. D., Gauthier, N. C., Biais, N., Lazar, A. M., Roca-Cusachs, P., Yu, C. H., & Sheetz, M. (2011). Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions. Molecular Biology of the Cell, 22(8), 1263-1273. https://doi.org/10.1091/mbc.E10-08-0661

Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions. / Lynch, Christopher D.; Gauthier, Nils C.; Biais, Nicolas; Lazar, Andre M.; Roca-Cusachs, Pere; Yu, Cheng Han; Sheetz, Michael.

In: Molecular Biology of the Cell, Vol. 22, No. 8, 15.04.2011, p. 1263-1273.

Research output: Contribution to journalArticle

Lynch, CD, Gauthier, NC, Biais, N, Lazar, AM, Roca-Cusachs, P, Yu, CH & Sheetz, M 2011, 'Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions', Molecular Biology of the Cell, vol. 22, no. 8, pp. 1263-1273. https://doi.org/10.1091/mbc.E10-08-0661
Lynch CD, Gauthier NC, Biais N, Lazar AM, Roca-Cusachs P, Yu CH et al. Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions. Molecular Biology of the Cell. 2011 Apr 15;22(8):1263-1273. https://doi.org/10.1091/mbc.E10-08-0661
Lynch, Christopher D. ; Gauthier, Nils C. ; Biais, Nicolas ; Lazar, Andre M. ; Roca-Cusachs, Pere ; Yu, Cheng Han ; Sheetz, Michael. / Filamin depletion blocks endoplasmic spreading and destabilizes force-bearing adhesions. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 8. pp. 1263-1273.
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