Filovirus-pseudotyped lentiviral vector can efficiently and stably transduce airway epithelia in vivo

Gary P. Kobinger, Daniel J. Weiner, Qian Chun Yu, James M. Wilson

Research output: Contribution to journalArticlepeer-review

264 Scopus citations

Abstract

Traditional gene therapy vectors have demonstrated limited utility for treatment of chronic lung diseases such as cystic fibrosis (CF). Herein we describe a vector based on a Filovirus envelope protein-pseudotyped HIV vector, which we chose after systematically evaluating multiple strategies. The vector efficiently transduces intact airway epithelium from the apical surface, as demonstrated in both in vitro and in vivo model systems. This shows the potential of pseudotyping in expanding the utility of lentiviral vectors. Pseudotyped lentiviral vectors may hold promise for the treatment of CF.

Original languageEnglish (US)
Pages (from-to)225-230
Number of pages6
JournalNature Biotechnology
Volume19
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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