First Biochemical Characterization of a Methylcitric Acid Cycle from Bacillus subtilis Strain 168

Jason J. Reddick, Sherona Sirkisoon, Rejwi Acharya Dahal, Grant Hardesty, Natalie E. Hage, William T. Booth, Amy L. Quattlebaum, Suzette N. Mills, Victoria G. Meadows, Sydney L.H. Adams, Jennifer S. Doyle, Brittany E. Kiel

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The genome of Bacillus subtilis strain 168 contains the mother cell metabolic gene (mmg) operon that encodes homologues from the methylcitric acid cycle. We showed that the three genes, mmgDE and yqiQ(mmgF), provide three of the five steps of the methylcitric acid cycle. We also showed that the fourth step can be supplied by citB (aconitase), and we suggest that the fifth missing step, the propionyl-CoA synthetase, is probably skipped because the β-oxidation of methyl-branched fatty acids by the enzymes encoded by mmgABC should produce propionyl-CoA. We also noted interesting enzymology for MmgD and MmgE. First, MmgD is a bifunctional citrate synthase/2-methylcitrate synthase with 2.3-fold higher activity as a 2-methylcitrate synthase. This enzyme catalyzes the formation of either (2S,3R)- or (2R,3S)-2-methylcitrate, but reports of 2-methylcitrate synthases from other species indicated that they produced the (2S,3S) isomer. However, we showed that MmgD and PrpC (from Escherichia coli) in fact produce the same stereoisomer. Second, the MmgE enzyme is not a stereospecific 2-methylcitrate dehydratase because it can dehydrate at least two of the four diastereomers of 2-methylcitrate to yield either (E)-2-methylaconitate or (Z)-2-methylaconitate. We also showed for the first time that the E. coli homologue PrpD exhibited the same lack of stereospecificity. However, the physiological pathways proceed via (Z)-2-methylaconitate, which served as the substrate for the citB enzyme in the synthesis of 2-methylisocitrate. We completed our characterization of this pathway by showing that the 2-methylisocitrate produced by CitB is converted to pyruvate and succinate by the enzyme YqiQ(MmgF).

Original languageEnglish (US)
Pages (from-to)5698-5711
Number of pages14
JournalBiochemistry
Volume56
Issue number42
DOIs
StatePublished - Oct 24 2017
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Reddick, J. J., Sirkisoon, S., Dahal, R. A., Hardesty, G., Hage, N. E., Booth, W. T., Quattlebaum, A. L., Mills, S. N., Meadows, V. G., Adams, S. L. H., Doyle, J. S., & Kiel, B. E. (2017). First Biochemical Characterization of a Methylcitric Acid Cycle from Bacillus subtilis Strain 168. Biochemistry, 56(42), 5698-5711. https://doi.org/10.1021/acs.biochem.7b00778