First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil

Vivaldo Gomes da Costa; Marielena Vogel Saivish; Priscila Gomes de Oliveira; Abelardo Silva-Júnior; Marcos Lázaro Moreli; Ricardo Henrique Krüger

doi:10.1038/s41598-021-92183-2

First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil

Vivaldo Gomes da Costa
, Marielena Vogel Saivish
, Priscila Gomes de Oliveira
, Abelardo Silva-Júnior
, Marcos Lázaro Moreli
, Ricardo Henrique Krüger

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]. However, little is known regarding the genetic variability of the strain circulating in several Brazilian regions. Here, we report the first full-length genome and molecular characterization of CDV isolated from domestic dogs in the Brazilian Center-West region. Sequence alignment and phylogenetic analyses based on deduced amino acid and nucleotide sequences showed that the isolated strain is characterized as the South America-I/Europe genotype. However, it segregates into a CDV subgenotype branch. Interestingly, both H and F proteins have a gain of a potential N-glycosylation sites compared to the Onderstepoort vaccine strain. Therefore, this study provides a reference to further understand the epidemic and molecular characteristics of the CDV in Brazil.

Original language	English (US)
Pages (from-to)	13039
Journal	Scientific reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-92183-2
State	Published - Jun 22 2021
Externally published	Yes

Keywords

Amino Acid Sequence
Animals
Base Sequence
Brazil
Distemper/virology
Distemper Virus, Canine/genetics
Dogs/virology
Genes, Viral
Genome, Viral
Genotype
Glycosylation
Phylogeny
Recombination, Genetic/genetics
Selection, Genetic
Viral Proteins/chemistry

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10.1038/s41598-021-92183-2

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title = "First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil",

abstract = "The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]. However, little is known regarding the genetic variability of the strain circulating in several Brazilian regions. Here, we report the first full-length genome and molecular characterization of CDV isolated from domestic dogs in the Brazilian Center-West region. Sequence alignment and phylogenetic analyses based on deduced amino acid and nucleotide sequences showed that the isolated strain is characterized as the South America-I/Europe genotype. However, it segregates into a CDV subgenotype branch. Interestingly, both H and F proteins have a gain of a potential N-glycosylation sites compared to the Onderstepoort vaccine strain. Therefore, this study provides a reference to further understand the epidemic and molecular characteristics of the CDV in Brazil.",

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author = "\{da Costa\}, \{Vivaldo Gomes\} and Saivish, \{Marielena Vogel\} and \{de Oliveira\}, \{Priscila Gomes\} and Abelardo Silva-J{\'u}nior and Moreli, \{Marcos L{\'a}zaro\} and Kr{\"u}ger, \{Ricardo Henrique\}",

year = "2021",

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day = "22",

doi = "10.1038/s41598-021-92183-2",

language = "English (US)",

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T1 - First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil

AU - da Costa, Vivaldo Gomes

AU - Saivish, Marielena Vogel

AU - de Oliveira, Priscila Gomes

AU - Silva-Júnior, Abelardo

AU - Moreli, Marcos Lázaro

AU - Krüger, Ricardo Henrique

PY - 2021/6/22

Y1 - 2021/6/22

N2 - The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]. However, little is known regarding the genetic variability of the strain circulating in several Brazilian regions. Here, we report the first full-length genome and molecular characterization of CDV isolated from domestic dogs in the Brazilian Center-West region. Sequence alignment and phylogenetic analyses based on deduced amino acid and nucleotide sequences showed that the isolated strain is characterized as the South America-I/Europe genotype. However, it segregates into a CDV subgenotype branch. Interestingly, both H and F proteins have a gain of a potential N-glycosylation sites compared to the Onderstepoort vaccine strain. Therefore, this study provides a reference to further understand the epidemic and molecular characteristics of the CDV in Brazil.

AB - The Brazilian regions are still highly endemic areas for Canine morbillivirus [canine distemper virus (CDV)]. However, little is known regarding the genetic variability of the strain circulating in several Brazilian regions. Here, we report the first full-length genome and molecular characterization of CDV isolated from domestic dogs in the Brazilian Center-West region. Sequence alignment and phylogenetic analyses based on deduced amino acid and nucleotide sequences showed that the isolated strain is characterized as the South America-I/Europe genotype. However, it segregates into a CDV subgenotype branch. Interestingly, both H and F proteins have a gain of a potential N-glycosylation sites compared to the Onderstepoort vaccine strain. Therefore, this study provides a reference to further understand the epidemic and molecular characteristics of the CDV in Brazil.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Brazil

KW - Distemper/virology

KW - Distemper Virus, Canine/genetics

KW - Dogs/virology

KW - Genes, Viral

KW - Genome, Viral

KW - Genotype

KW - Glycosylation

KW - Phylogeny

KW - Recombination, Genetic/genetics

KW - Selection, Genetic

KW - Viral Proteins/chemistry

U2 - 10.1038/s41598-021-92183-2

DO - 10.1038/s41598-021-92183-2

M3 - Article

C2 - 34158515

SN - 2045-2322

VL - 11

SP - 13039

JO - Scientific reports

JF - Scientific reports

IS - 1

ER -

First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil

Abstract

Keywords

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