First detection of chlamydia trachomatis 'Swedish' variant (nvCT) in a Russian couple with infertility

Valentina Feodorova, Edgar Sultanakhmedov, Yury Saltykov, Sergey Zaitsev, Sergey Utz, Michael Corbel, Charlotte Gaydos, Thomas Quinn, Vladimir Motin

Research output: Contribution to journalArticle

Abstract

Background: Chronic asymptomatic chlamydial genital infection caused by the wild-type of Chlamydia trachomatis (wtCT) is the most common bacterial infection causing human infertility. The novel ‘Swedish’ variant of С.trachomatis (nvCT) which contains a 377 bp deletion in a region that is specifically targeted in some nucleic acid amplification tests may impede diagnosis. Objective: The study aimed to investigate whether nvCT may be a possible cause of infertility in a couple undergoing in vitro fertilization (IVF). Method: Clinical specimens from both genital (urethra and cervix) and extra-genital sites (pharynx, conjunctive, blood) of a couple who experienced multiple unsuccessful attempts at pregnancy by natural fertilization and IVF procedures were analyzed before and after antibiotic therapy. Both partners had neither somatic nor endocrinal abnormality nor any clinically apparent genital manifestations of Chlamydia or other STIs. Results: Before antibiotic therapy all the samples of the Female Partner (FP) contained DNA of only the nvCT. After antibiotic therapy, additionally, DNA of wtCT of genovars E and D was detected in specimens from her conjunctiva and oropharynx. All samples of the Male Partner (MP) revealed co-infection of nvCT and wtCT. Identical SNP within the variable region 4 (VD4) of the ompA gene confirmed the identity of the wtCT strains found in both partners. The FP had a positive anti-chlamydial IgG titer. The sperm characteristics of the MP, motility (immotile spermatozoa was 51.1% versus 21.6%) and vitality (46% versus 68%) declined progressively, and the MP anti-chlamydial IgG titer was negative. Conclusion: Infertility in this couple may have been caused by chronic asymptomatic and persistent nvCT-associated infection that was complicated by re-infection later with wtCT. This study illustrates the importance of including detection methods for nvCT strains in the investigation of infertility cases.

Original languageEnglish (US)
Pages (from-to)343-352
Number of pages10
JournalOpen Microbiology Journal
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2018

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Chlamydia trachomatis
Infertility
Fertilization in Vitro
Anti-Bacterial Agents
Spermatozoa
Infection
Nucleic Acid Amplification Techniques
Oropharynx
Chlamydia
Conjunctiva
DNA
Urethra
Sexually Transmitted Diseases
Pharynx
Coinfection
Bacterial Infections
Cervix Uteri
Single Nucleotide Polymorphism
Therapeutics
Pregnancy

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

First detection of chlamydia trachomatis 'Swedish' variant (nvCT) in a Russian couple with infertility. / Feodorova, Valentina; Sultanakhmedov, Edgar; Saltykov, Yury; Zaitsev, Sergey; Utz, Sergey; Corbel, Michael; Gaydos, Charlotte; Quinn, Thomas; Motin, Vladimir.

In: Open Microbiology Journal, Vol. 12, No. 1, 01.01.2018, p. 343-352.

Research output: Contribution to journalArticle

Feodorova, V, Sultanakhmedov, E, Saltykov, Y, Zaitsev, S, Utz, S, Corbel, M, Gaydos, C, Quinn, T & Motin, V 2018, 'First detection of chlamydia trachomatis 'Swedish' variant (nvCT) in a Russian couple with infertility', Open Microbiology Journal, vol. 12, no. 1, pp. 343-352. https://doi.org/10.2174/1874285801812010343
Feodorova, Valentina ; Sultanakhmedov, Edgar ; Saltykov, Yury ; Zaitsev, Sergey ; Utz, Sergey ; Corbel, Michael ; Gaydos, Charlotte ; Quinn, Thomas ; Motin, Vladimir. / First detection of chlamydia trachomatis 'Swedish' variant (nvCT) in a Russian couple with infertility. In: Open Microbiology Journal. 2018 ; Vol. 12, No. 1. pp. 343-352.
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abstract = "Background: Chronic asymptomatic chlamydial genital infection caused by the wild-type of Chlamydia trachomatis (wtCT) is the most common bacterial infection causing human infertility. The novel ‘Swedish’ variant of С.trachomatis (nvCT) which contains a 377 bp deletion in a region that is specifically targeted in some nucleic acid amplification tests may impede diagnosis. Objective: The study aimed to investigate whether nvCT may be a possible cause of infertility in a couple undergoing in vitro fertilization (IVF). Method: Clinical specimens from both genital (urethra and cervix) and extra-genital sites (pharynx, conjunctive, blood) of a couple who experienced multiple unsuccessful attempts at pregnancy by natural fertilization and IVF procedures were analyzed before and after antibiotic therapy. Both partners had neither somatic nor endocrinal abnormality nor any clinically apparent genital manifestations of Chlamydia or other STIs. Results: Before antibiotic therapy all the samples of the Female Partner (FP) contained DNA of only the nvCT. After antibiotic therapy, additionally, DNA of wtCT of genovars E and D was detected in specimens from her conjunctiva and oropharynx. All samples of the Male Partner (MP) revealed co-infection of nvCT and wtCT. Identical SNP within the variable region 4 (VD4) of the ompA gene confirmed the identity of the wtCT strains found in both partners. The FP had a positive anti-chlamydial IgG titer. The sperm characteristics of the MP, motility (immotile spermatozoa was 51.1{\%} versus 21.6{\%}) and vitality (46{\%} versus 68{\%}) declined progressively, and the MP anti-chlamydial IgG titer was negative. Conclusion: Infertility in this couple may have been caused by chronic asymptomatic and persistent nvCT-associated infection that was complicated by re-infection later with wtCT. This study illustrates the importance of including detection methods for nvCT strains in the investigation of infertility cases.",
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