Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells

Xin Zhou, Jorge A. Girón, Alfredo Torres, J. Adam Crawford, Erasmo Negrete, Stefanie N. Vogel, James B. Kaper

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The type III secretion system (TTSS) of enteropathogenic Escherichia coli (EPEC) has been associated with the ability of these bacteria to induce secretion of proinflammatory cytokines, including interleukin-8 (IL-8), in cultured epithelial cells. However, the identity of the effector molecule directly involved in this event is unknown. In this study, we determined that the native flagellar filament and its flagellin monomer are activators of IL-8 release in T84 epithelial cells. Supernatants of wild-type EPEC strain E2348/69 and its isogenic mutants deficient in TTSS (escN) and in production of intimin (eae), grown in Luria-Bertani broth, elicited similar amounts of IL-8 secretion by T84 cells. In contrast, supernatants of EPEC fliC mutants and of B171, a nonflagellated EPEC strain, were defective in inducing IL-8 release, a phenotype that was largely restored by complementation of the fliC gene in the mutant lacking flagella. Purified flagella from E. coli K-12, EPEC serotypes H6 and H34, and enterohemorrhagic E. coli serotype H7 all induced IL-8 release in T84 cells. Induction of IL-8 by purified flagella or His-tagged FliC from EPEC strain E2348/69 was dose dependent and was blocked by a polyclonal anti-H6 antibody. Finally, the mitogen-activated protein kinases (Erk1 and -2 and Jnk) were phosphorylated in flagellin-treated T84 cells, and inhibition of the p38 and Erk pathways significantly decreased the IL-8 response induced by EPEC flagellin. Our data clearly indicate that FliC of EPEC is sufficient to induce IL-8 release in T84 cells and that activation of the Erk and p38 pathways is required for IL-8 induction.

Original languageEnglish (US)
Pages (from-to)2120-2129
Number of pages10
JournalInfection and Immunity
Volume71
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

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Flagellin
Enteropathogenic Escherichia coli
Interleukin-8
Flagella
Epithelial Cells
Enterohemorrhagic Escherichia coli
Mitogen-Activated Protein Kinase 1
Anti-Idiotypic Antibodies
Cultured Cells
Cytokines
Escherichia coli
Bacteria
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells. / Zhou, Xin; Girón, Jorge A.; Torres, Alfredo; Crawford, J. Adam; Negrete, Erasmo; Vogel, Stefanie N.; Kaper, James B.

In: Infection and Immunity, Vol. 71, No. 4, 01.04.2003, p. 2120-2129.

Research output: Contribution to journalArticle

Zhou, Xin ; Girón, Jorge A. ; Torres, Alfredo ; Crawford, J. Adam ; Negrete, Erasmo ; Vogel, Stefanie N. ; Kaper, James B. / Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells. In: Infection and Immunity. 2003 ; Vol. 71, No. 4. pp. 2120-2129.
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AU - Girón, Jorge A.

AU - Torres, Alfredo

AU - Crawford, J. Adam

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AU - Vogel, Stefanie N.

AU - Kaper, James B.

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