Flaviviral replication complex: Coordination between RNA synthesis and 5'-RNA capping

Valerie J. Klema, Radhakrishnan Padmanabhan, Kyung Choi

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Genome replication in flavivirus requires (-) strand RNA synthesis, (+) strand RNA synthesis, and 5'-RNA capping and methylation. To carry out viral genome replication, flavivirus assembles a replication complex, consisting of both viral and host proteins, on the cytoplasmic side of the endoplasmic reticulum (ER) membrane. Two major components of the replication complex are the viral non-structural (NS) proteins NS3 and NS5. Together they possess all the enzymatic activities required for genome replication, yet how these activities are coordinated during genome replication is not clear. We provide an overview of the flaviviral genome replication process, the membrane-bound replication complex, and recent crystal structures of full-length NS5. We propose a model of how NS3 and NS5 coordinate their activities in the individual steps of (-) RNA synthesis, (+) RNA synthesis, and 5'-RNA capping and methylation.

Original languageEnglish (US)
Pages (from-to)4640-4656
Number of pages17
JournalViruses
Volume7
Issue number8
DOIs
StatePublished - Aug 13 2015

Keywords

  • 5'-RNA capping
  • Flavivirus
  • NS3
  • NS5
  • RNA synthesis
  • RNA-dependent RNA polymerase
  • Viral replication complex

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Fingerprint Dive into the research topics of 'Flaviviral replication complex: Coordination between RNA synthesis and 5'-RNA capping'. Together they form a unique fingerprint.

  • Cite this