Flavivirus-induced antibody cross-reactivity

Karen L. Mansfield, Daniel L. Horton, Nicholas Johnson, Li Li, Alan Barrett, Derek J. Smith, Sareen E. Galbraith, Tom Solomon, Anthony R. Fooks

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Dengue viruses (DENV) cause countless human deaths each year, whilst West Nile virus (WNV) has re-emerged as an important human pathogen. There are currently no WNV or DENV vaccines licensed for human use, yet vaccines exist against other flaviviruses. To investigate flavivirus cross-reactivity, sera from a human cohort with a history of vaccination against tick-borne encephalitis virus (TBEV), Japanese encephalitis virus (JEV) and yellow fever virus (YFV) were tested for antibodies by plaque reduction neutralization test. Neutralization of louping ill virus (LIV) occurred, but no significant neutralization of Murray Valley encephalitis virus was observed. Sera from some individuals vaccinated against TBEV and JEV neutralized WNV, which was enhanced by YFV vaccination in some recipients. Similarly, some individuals neutralized DENV-2, but this was not significantly influenced by YFV vaccination. Antigenic cartography techniques were used to generate a geometric illustration of the neutralization titres of selected sera against WNV, TBEV, JEV, LIV, YFV and DENV-2. This demonstrated the individual variation in antibody responses. Most sera had detectable titres against LIV and some had titres against WNV and DENV-2. Generally, LIV titres were similar to titres against TBEV, confirming the close antigenic relationship between TBEV and LIV. JEV was also antigenically closer to TBEV than WNV, using these sera. The use of sera from individuals vaccinated against multiple pathogens is unique relative to previous applications of antigenic cartography techniques. It is evident from these data that notable differences exist between amino acid sequence identity and mapped antigenic relationships within the family Flaviviridae.

Original languageEnglish (US)
Pages (from-to)2821-2829
Number of pages9
JournalJournal of General Virology
Volume92
Issue number12
DOIs
StatePublished - Dec 2011

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Tick-Borne Encephalitis Viruses
Flavivirus
West Nile virus
Japanese Encephalitis Virus
Antibodies
Dengue Virus
Yellow fever virus
Serum
Vaccination
Murray Valley encephalitis virus
Dengue Vaccines
Flaviviridae
Neutralization Tests
Viral Load
Antibody Formation
Amino Acid Sequence
Vaccines

ASJC Scopus subject areas

  • Virology

Cite this

Mansfield, K. L., Horton, D. L., Johnson, N., Li, L., Barrett, A., Smith, D. J., ... Fooks, A. R. (2011). Flavivirus-induced antibody cross-reactivity. Journal of General Virology, 92(12), 2821-2829. https://doi.org/10.1099/vir.0.031641-0

Flavivirus-induced antibody cross-reactivity. / Mansfield, Karen L.; Horton, Daniel L.; Johnson, Nicholas; Li, Li; Barrett, Alan; Smith, Derek J.; Galbraith, Sareen E.; Solomon, Tom; Fooks, Anthony R.

In: Journal of General Virology, Vol. 92, No. 12, 12.2011, p. 2821-2829.

Research output: Contribution to journalArticle

Mansfield, KL, Horton, DL, Johnson, N, Li, L, Barrett, A, Smith, DJ, Galbraith, SE, Solomon, T & Fooks, AR 2011, 'Flavivirus-induced antibody cross-reactivity', Journal of General Virology, vol. 92, no. 12, pp. 2821-2829. https://doi.org/10.1099/vir.0.031641-0
Mansfield KL, Horton DL, Johnson N, Li L, Barrett A, Smith DJ et al. Flavivirus-induced antibody cross-reactivity. Journal of General Virology. 2011 Dec;92(12):2821-2829. https://doi.org/10.1099/vir.0.031641-0
Mansfield, Karen L. ; Horton, Daniel L. ; Johnson, Nicholas ; Li, Li ; Barrett, Alan ; Smith, Derek J. ; Galbraith, Sareen E. ; Solomon, Tom ; Fooks, Anthony R. / Flavivirus-induced antibody cross-reactivity. In: Journal of General Virology. 2011 ; Vol. 92, No. 12. pp. 2821-2829.
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