Flavivirus RNA synthesis in vitro

Radhakrishnan Padmanabhan; Ratree Takhampunya; Tadahisa Teramoto; Kyung Choi

doi:10.1016/j.ymeth.2015.08.002

Flavivirus RNA synthesis in vitro

Radhakrishnan Padmanabhan, Ratree Takhampunya, Tadahisa Teramoto, Kyung Choi

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.

Original language	English (US)
Journal	Methods
DOIs	https://doi.org/10.1016/j.ymeth.2015.08.002
State	Accepted/In press - Jun 3 2015

Keywords

Cytoplasmic membrane-bound viral replication complex
Endogenous and exogenous viral RNA templates
Heparin trap
Polarity of RNA product by RNase H mapping
RNA-dependent RNA polymerase from positive strand mosquito-borne Flaviviridae
Temperature-dependent de novo initiation and elongation

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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title = "Flavivirus RNA synthesis in vitro",

abstract = "Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.",

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AU - Takhampunya, Ratree

AU - Teramoto, Tadahisa

AU - Choi, Kyung

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