Flavivirus RNA synthesis in vitro

Radhakrishnan Padmanabhan, Ratree Takhampunya, Tadahisa Teramoto, Kyung Choi

    Research output: Contribution to journal › Article

    4 Citations (Scopus)

    Abstract

    Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.

    Original languageEnglish (US)
    JournalMethods
    DOIs
    StateAccepted/In press - Jun 3 2015

    Fingerprint

    Flavivirus
    RNA
    RNA Replicase
    Trans-Activators
    RNA Viruses
    Proteins
    Viruses
    Membranes
    In Vitro Techniques
    Chemical analysis

    Keywords

    • Cytoplasmic membrane-bound viral replication complex
    • Endogenous and exogenous viral RNA templates
    • Heparin trap
    • Polarity of RNA product by RNase H mapping
    • RNA-dependent RNA polymerase from positive strand mosquito-borne Flaviviridae
    • Temperature-dependent de novo initiation and elongation

    ASJC Scopus subject areas

    • Molecular Biology
    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Padmanabhan, R., Takhampunya, R., Teramoto, T., & Choi, K. (Accepted/In press). Flavivirus RNA synthesis in vitro. Methods. https://doi.org/10.1016/j.ymeth.2015.08.002

    Flavivirus RNA synthesis in vitro. / Padmanabhan, Radhakrishnan; Takhampunya, Ratree; Teramoto, Tadahisa; Choi, Kyung.

    In: Methods, 03.06.2015.

    Research output: Contribution to journal › Article

    Padmanabhan, R, Takhampunya, R, Teramoto, T & Choi, K 2015, 'Flavivirus RNA synthesis in vitro', Methods. https://doi.org/10.1016/j.ymeth.2015.08.002
    Padmanabhan R, Takhampunya R, Teramoto T, Choi K. Flavivirus RNA synthesis in vitro. Methods. 2015 Jun 3. https://doi.org/10.1016/j.ymeth.2015.08.002
    Padmanabhan, Radhakrishnan ; Takhampunya, Ratree ; Teramoto, Tadahisa ; Choi, Kyung. / Flavivirus RNA synthesis in vitro. In: Methods. 2015.
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