TY - JOUR
T1 - Flavivirus RNA synthesis in vitro
AU - Padmanabhan, Radhakrishnan
AU - Takhampunya, Ratree
AU - Teramoto, Tadahisa
AU - Choi, Kyung H.
N1 - Funding Information:
The studies in authors’ laboratories described in this review are supported by NIAID/NIH grants AI32078 (RP), AI 54776 (CEC and RP), and AI087856 (K. H. C and R. P). We thank Dr. Rajendra Pilankatta for critical reading of the manuscript.
Funding Information:
The studies in authors’ laboratories described in this review are supported by NIAID/NIH grants AI32078 (RP), AI 54776 (CEC and RP), and AI087856 (K. H. C and R. P). We thank Dr. Rajendra Pilankatta for critical reading of the manuscript.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015
Y1 - 2015
N2 - Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.
AB - Establishment of in vitro systems to study mechanisms of RNA synthesis for positive strand RNA viruses have been very useful in the past and have shed light on the composition of protein and RNA components, optimum conditions, the nature of the products formed, cis-acting RNA elements and trans-acting protein factors required for efficient synthesis. In this review, we summarize our current understanding regarding the requirements for flavivirus RNA synthesis in vitro. We describe details of reaction conditions, the specificity of template used by either the multi-component membrane-bound viral replicase complex or by purified, recombinant RNA-dependent RNA polymerase. We also discuss future perspectives to extend the boundaries of our knowledge.
KW - Cytoplasmic membrane-bound viral replication complex
KW - Endogenous and exogenous viral RNA templates
KW - Heparin trap
KW - Polarity of RNA product by RNase H mapping
KW - RNA-dependent RNA polymerase from positive strand mosquito-borne Flaviviridae
KW - Temperature-dependent de novo initiation and elongation
UR - http://www.scopus.com/inward/record.url?scp=84940094335&partnerID=8YFLogxK
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U2 - 10.1016/j.ymeth.2015.08.002
DO - 10.1016/j.ymeth.2015.08.002
M3 - Review article
C2 - 26272247
AN - SCOPUS:84940094335
VL - 91
SP - 20
EP - 34
JO - ImmunoMethods
JF - ImmunoMethods
SN - 1046-2023
ER -