Flexor tendon tissue engineering: Bioreactor cyclic strain increases construct strength

Sepideh Saber, Andrew Y. Zhang, Sae H. Ki, Derek P. Lindsey, Robert Lane Smith, Jonathan Riboh, Hung Pham, James Chang

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Mutilating injuries of the hand and upper extremity result in tendon losses too great to be replaced by autologous grafts. The goal of this study was to use tissue engineering techniques to produce additional tendon material. We used a custom bioreactor to apply cyclic mechanical loading onto tissue-engineered tendon constructs to study ultimate tensile stress (UTS) and elastic modulus (E). Constructs used were acellularized rabbit hindpaw flexor digitorum profundus equivalents reseeded with tenocytes or left unseeded. Tendon constructs were subjected to a stretch force of 1.25 N over a 5-day course. Seeded tendon constructs that were exposed to bioreactor loading had a significantly increased UTS (71.17 ± 14.15 N) compared to nonloaded controls (35.69 ± 5.62 N) (p = 0.001). Similarly, seeded constructs exposed to bioreactor loading also had a significantly higher E (1091 ± 169 MPa) compared to nonloaded controls (632 ± 86 MPa) (p = 0.001). This study shows that cyclic loading of tendon constructs increases the UTS and elastic modulus of seeded constructs. The use of the bioreactor may therefore accelerate the in vitro production of strong, nonimmunogenic tendon material that can potentially be used clinically to reconstruct significant tendon losses.

Original languageEnglish (US)
Pages (from-to)2085-2090
Number of pages6
JournalTissue Engineering - Part A
Volume16
Issue number6
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Tendons
Bioreactors
Tissue Engineering
Tissue engineering
Tensile stress
Elastic Modulus
Elastic moduli
Hand Injuries
Grafts
Upper Extremity
Tissue
Rabbits
Transplants

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Saber, S., Zhang, A. Y., Ki, S. H., Lindsey, D. P., Smith, R. L., Riboh, J., ... Chang, J. (2010). Flexor tendon tissue engineering: Bioreactor cyclic strain increases construct strength. Tissue Engineering - Part A, 16(6), 2085-2090. https://doi.org/10.1089/ten.tea.2010.0032

Flexor tendon tissue engineering : Bioreactor cyclic strain increases construct strength. / Saber, Sepideh; Zhang, Andrew Y.; Ki, Sae H.; Lindsey, Derek P.; Smith, Robert Lane; Riboh, Jonathan; Pham, Hung; Chang, James.

In: Tissue Engineering - Part A, Vol. 16, No. 6, 2010, p. 2085-2090.

Research output: Contribution to journalArticle

Saber, S, Zhang, AY, Ki, SH, Lindsey, DP, Smith, RL, Riboh, J, Pham, H & Chang, J 2010, 'Flexor tendon tissue engineering: Bioreactor cyclic strain increases construct strength', Tissue Engineering - Part A, vol. 16, no. 6, pp. 2085-2090. https://doi.org/10.1089/ten.tea.2010.0032
Saber, Sepideh ; Zhang, Andrew Y. ; Ki, Sae H. ; Lindsey, Derek P. ; Smith, Robert Lane ; Riboh, Jonathan ; Pham, Hung ; Chang, James. / Flexor tendon tissue engineering : Bioreactor cyclic strain increases construct strength. In: Tissue Engineering - Part A. 2010 ; Vol. 16, No. 6. pp. 2085-2090.
@article{3bed7b1c225746788f6229ac96d2d85d,
title = "Flexor tendon tissue engineering: Bioreactor cyclic strain increases construct strength",
abstract = "Mutilating injuries of the hand and upper extremity result in tendon losses too great to be replaced by autologous grafts. The goal of this study was to use tissue engineering techniques to produce additional tendon material. We used a custom bioreactor to apply cyclic mechanical loading onto tissue-engineered tendon constructs to study ultimate tensile stress (UTS) and elastic modulus (E). Constructs used were acellularized rabbit hindpaw flexor digitorum profundus equivalents reseeded with tenocytes or left unseeded. Tendon constructs were subjected to a stretch force of 1.25 N over a 5-day course. Seeded tendon constructs that were exposed to bioreactor loading had a significantly increased UTS (71.17 ± 14.15 N) compared to nonloaded controls (35.69 ± 5.62 N) (p = 0.001). Similarly, seeded constructs exposed to bioreactor loading also had a significantly higher E (1091 ± 169 MPa) compared to nonloaded controls (632 ± 86 MPa) (p = 0.001). This study shows that cyclic loading of tendon constructs increases the UTS and elastic modulus of seeded constructs. The use of the bioreactor may therefore accelerate the in vitro production of strong, nonimmunogenic tendon material that can potentially be used clinically to reconstruct significant tendon losses.",
author = "Sepideh Saber and Zhang, {Andrew Y.} and Ki, {Sae H.} and Lindsey, {Derek P.} and Smith, {Robert Lane} and Jonathan Riboh and Hung Pham and James Chang",
year = "2010",
doi = "10.1089/ten.tea.2010.0032",
language = "English (US)",
volume = "16",
pages = "2085--2090",
journal = "Tissue Engineering - Part A.",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "6",

}

TY - JOUR

T1 - Flexor tendon tissue engineering

T2 - Bioreactor cyclic strain increases construct strength

AU - Saber, Sepideh

AU - Zhang, Andrew Y.

AU - Ki, Sae H.

AU - Lindsey, Derek P.

AU - Smith, Robert Lane

AU - Riboh, Jonathan

AU - Pham, Hung

AU - Chang, James

PY - 2010

Y1 - 2010

N2 - Mutilating injuries of the hand and upper extremity result in tendon losses too great to be replaced by autologous grafts. The goal of this study was to use tissue engineering techniques to produce additional tendon material. We used a custom bioreactor to apply cyclic mechanical loading onto tissue-engineered tendon constructs to study ultimate tensile stress (UTS) and elastic modulus (E). Constructs used were acellularized rabbit hindpaw flexor digitorum profundus equivalents reseeded with tenocytes or left unseeded. Tendon constructs were subjected to a stretch force of 1.25 N over a 5-day course. Seeded tendon constructs that were exposed to bioreactor loading had a significantly increased UTS (71.17 ± 14.15 N) compared to nonloaded controls (35.69 ± 5.62 N) (p = 0.001). Similarly, seeded constructs exposed to bioreactor loading also had a significantly higher E (1091 ± 169 MPa) compared to nonloaded controls (632 ± 86 MPa) (p = 0.001). This study shows that cyclic loading of tendon constructs increases the UTS and elastic modulus of seeded constructs. The use of the bioreactor may therefore accelerate the in vitro production of strong, nonimmunogenic tendon material that can potentially be used clinically to reconstruct significant tendon losses.

AB - Mutilating injuries of the hand and upper extremity result in tendon losses too great to be replaced by autologous grafts. The goal of this study was to use tissue engineering techniques to produce additional tendon material. We used a custom bioreactor to apply cyclic mechanical loading onto tissue-engineered tendon constructs to study ultimate tensile stress (UTS) and elastic modulus (E). Constructs used were acellularized rabbit hindpaw flexor digitorum profundus equivalents reseeded with tenocytes or left unseeded. Tendon constructs were subjected to a stretch force of 1.25 N over a 5-day course. Seeded tendon constructs that were exposed to bioreactor loading had a significantly increased UTS (71.17 ± 14.15 N) compared to nonloaded controls (35.69 ± 5.62 N) (p = 0.001). Similarly, seeded constructs exposed to bioreactor loading also had a significantly higher E (1091 ± 169 MPa) compared to nonloaded controls (632 ± 86 MPa) (p = 0.001). This study shows that cyclic loading of tendon constructs increases the UTS and elastic modulus of seeded constructs. The use of the bioreactor may therefore accelerate the in vitro production of strong, nonimmunogenic tendon material that can potentially be used clinically to reconstruct significant tendon losses.

UR - http://www.scopus.com/inward/record.url?scp=77953350369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953350369&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2010.0032

DO - 10.1089/ten.tea.2010.0032

M3 - Article

C2 - 20109062

AN - SCOPUS:77953350369

VL - 16

SP - 2085

EP - 2090

JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

SN - 1937-3341

IS - 6

ER -