Fluid compartments in hemorrhaged rats after hyperosmotic crystalloid and hyperoncotic colloid resuscitation

Paula F. Moon, Michele A. Hollyfield-Gilbert, Tamara L. Myers, Tatsuo Uchida, George Kramer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Postresuscitation organ failure may be associated with detrimental changes in body fluid compartments. We measured how shock and resuscitation acutely alters the interstitial, cellular, and plasma compartments in different organs. Nephrectomized, anesthetized rats were bled to 50 mmHg mean arterial pressure for 1 h, followed by 60 min of resuscitation to restore blood pressure using 0.9% normal saline(NS, n = 10), 7.5% hypertonic saline(HS, n = 8), 10% hyperoncotic albumin (HA, n = 8), or 7.5% hypertonic saline and 10% hyperoncotic albumin (HSA, n = 7). A 2-h 51Cr-EDTA distribution space estimated extracellular fluid volume (ECFV), and a 5-min 125I-labeled albumin distribution space measured plasma volume (PV). Total tissue water (TW) was measured from wet and dry weights; interstitial fluid volume (ISFV) and cell water were calculated. NS resuscitation required 7 times more fluid (50.9 ± 7.7 vs. 8.6 ± 0.7 for HA, 5.9 ± 0.4 for HS, and 3.9 ± 0.5 ml/kg for HSA), but there were no differences between solutions in whole animal PV, ECFV, or ISFV. Fluid shifts within tissues depended on resuscitation solution and type of tissue. TW was significantly reduced by hypertonic saline groups in heart, muscle, and liver (P < 0.05). ISFV was significantly reduced by HA groups in the skin. In all tissues, mean cell water in groups receiving HS was smaller; this was significant for heart, lung, muscle, and skin. In conclusion, 1 ) HS solutions mobilize fluid from cells while expanding both PV and ISFV, and 2 ) TW and cellular water increase with both isotonic crystalloids and hyperoncotic colloids in many tissues.

Original languageEnglish (US)
JournalAmerican Journal of Physiology
Volume270
Issue number1 PART 2
StatePublished - 1996

Fingerprint

Colloids
Resuscitation
Extracellular Fluid
Water
Plasma Volume
Myocardium
Body Fluid Compartments
Fluid Shifts
Skin
crystalloid solutions
Cell Size
Edetic Acid
Albumins
Shock
Arterial Pressure
Blood Pressure
Weights and Measures
Lung
Liver

Keywords

  • Cellular edema
  • Fluid therapy
  • Hypertonic saline
  • Interstitial edema
  • Shock

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Fluid compartments in hemorrhaged rats after hyperosmotic crystalloid and hyperoncotic colloid resuscitation. / Moon, Paula F.; Hollyfield-Gilbert, Michele A.; Myers, Tamara L.; Uchida, Tatsuo; Kramer, George.

In: American Journal of Physiology, Vol. 270, No. 1 PART 2, 1996.

Research output: Contribution to journalArticle

Moon, Paula F. ; Hollyfield-Gilbert, Michele A. ; Myers, Tamara L. ; Uchida, Tatsuo ; Kramer, George. / Fluid compartments in hemorrhaged rats after hyperosmotic crystalloid and hyperoncotic colloid resuscitation. In: American Journal of Physiology. 1996 ; Vol. 270, No. 1 PART 2.
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