Fluorescence spectra of normal and atherosclerotic human aorta: Optimum discriminant analysis

Richard J. Crilly, Stephen Gunther, Massoud Motamedi, Ramon Berguer, J. Richard Spears

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To evaluate the usefulness of tissue fluorescence to distinguish normal from atherosclerotic regions in human arteries, we examined fluorescence spectral maps obtained over a wide range of excitation and emission wavelengths from 15 normal and 26 atherosclerotic human aorta specimens. After defining criteria to maximize safety, ease of use, reproducibility and predictive accuracy, we derived an optimum fluorescence discriminant function from this data set which reliably identifies normal and diseased arterial tissue, is independent of sampling device characteristics and vessel geometry, and utilizes simple, available methodologies. This discriminant function, the ratio of fluorescence intensity emitted at 380 nm to that emitted at 420 nm with excitation wavelength of 360 nm [I380/I420)360] compared favorably to other fluorescent discrimination techniques previously described.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1067
DOIs
StatePublished - Jun 15 1989
Externally publishedYes

Fingerprint

aorta
Aorta
Discriminant analysis
Discriminant Analysis
Fluorescence
Discriminant Function
fluorescence
Excitation
Wavelength
Tissue
samplers
Reproducibility
Arteries
arteries
wavelengths
Vessel
Discrimination
excitation
vessels
discrimination

ASJC Scopus subject areas

  • Applied Mathematics
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering
  • Computer Science Applications

Cite this

Fluorescence spectra of normal and atherosclerotic human aorta : Optimum discriminant analysis. / Crilly, Richard J.; Gunther, Stephen; Motamedi, Massoud; Berguer, Ramon; Spears, J. Richard.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1067, 15.06.1989, p. 110-115.

Research output: Contribution to journalArticle

@article{7cb6318cf2b84d56a02d9bea97d858e7,
title = "Fluorescence spectra of normal and atherosclerotic human aorta: Optimum discriminant analysis",
abstract = "To evaluate the usefulness of tissue fluorescence to distinguish normal from atherosclerotic regions in human arteries, we examined fluorescence spectral maps obtained over a wide range of excitation and emission wavelengths from 15 normal and 26 atherosclerotic human aorta specimens. After defining criteria to maximize safety, ease of use, reproducibility and predictive accuracy, we derived an optimum fluorescence discriminant function from this data set which reliably identifies normal and diseased arterial tissue, is independent of sampling device characteristics and vessel geometry, and utilizes simple, available methodologies. This discriminant function, the ratio of fluorescence intensity emitted at 380 nm to that emitted at 420 nm with excitation wavelength of 360 nm [I380/I420)360] compared favorably to other fluorescent discrimination techniques previously described.",
author = "Crilly, {Richard J.} and Stephen Gunther and Massoud Motamedi and Ramon Berguer and Spears, {J. Richard}",
year = "1989",
month = "6",
day = "15",
doi = "10.1117/12.952109",
language = "English (US)",
volume = "1067",
pages = "110--115",
journal = "Proceedings of SPIE - The International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

TY - JOUR

T1 - Fluorescence spectra of normal and atherosclerotic human aorta

T2 - Optimum discriminant analysis

AU - Crilly, Richard J.

AU - Gunther, Stephen

AU - Motamedi, Massoud

AU - Berguer, Ramon

AU - Spears, J. Richard

PY - 1989/6/15

Y1 - 1989/6/15

N2 - To evaluate the usefulness of tissue fluorescence to distinguish normal from atherosclerotic regions in human arteries, we examined fluorescence spectral maps obtained over a wide range of excitation and emission wavelengths from 15 normal and 26 atherosclerotic human aorta specimens. After defining criteria to maximize safety, ease of use, reproducibility and predictive accuracy, we derived an optimum fluorescence discriminant function from this data set which reliably identifies normal and diseased arterial tissue, is independent of sampling device characteristics and vessel geometry, and utilizes simple, available methodologies. This discriminant function, the ratio of fluorescence intensity emitted at 380 nm to that emitted at 420 nm with excitation wavelength of 360 nm [I380/I420)360] compared favorably to other fluorescent discrimination techniques previously described.

AB - To evaluate the usefulness of tissue fluorescence to distinguish normal from atherosclerotic regions in human arteries, we examined fluorescence spectral maps obtained over a wide range of excitation and emission wavelengths from 15 normal and 26 atherosclerotic human aorta specimens. After defining criteria to maximize safety, ease of use, reproducibility and predictive accuracy, we derived an optimum fluorescence discriminant function from this data set which reliably identifies normal and diseased arterial tissue, is independent of sampling device characteristics and vessel geometry, and utilizes simple, available methodologies. This discriminant function, the ratio of fluorescence intensity emitted at 380 nm to that emitted at 420 nm with excitation wavelength of 360 nm [I380/I420)360] compared favorably to other fluorescent discrimination techniques previously described.

UR - http://www.scopus.com/inward/record.url?scp=24344485610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24344485610&partnerID=8YFLogxK

U2 - 10.1117/12.952109

DO - 10.1117/12.952109

M3 - Article

AN - SCOPUS:24344485610

VL - 1067

SP - 110

EP - 115

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -