Fluorinated amphiphilic amino acid derivatives as antioxidant carriers: A new class of protective agents

Stéphanie Ortial, Grégory Durand, Burkhard Poeggeler, Ange Polidori, Miguel Pappolla, Jutta Böker, Rüdiger Hardeland, Bernard Pucci

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40 Scopus citations

Abstract

The use of classical antioxidants is limited by their low bioavailabilities, and therefore, high doses are usually required to display significant protective activity. In a recent article (J. Med. Chem. 2003, 46, 5230) we showed that the ability of the α-phenyl-N-tert-butylnitrone (PBN) to restore the viability of ATPase-deficient human skin fibroblasts was greatly enhanced by grafting it on a fluorinated amphiphilic carrier. With the aim of extending this concept to other antioxidants, we present here the design, the synthesis, and the physicochemical measurements of a new series of fluorinated amphiphilic antioxidant derivatives. The hydroxyl radical scavenging activity and the radical reducing potency of these newly designed compounds were respectively demonstrated in an ABTS competition and an ABTS.+ reduction assay. We also showed that the protective effects of amphiphilic antioxidants derived from PBN, Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2- carboxylic acid) or lipoic acid (5-[1,2]-dithiolan-3-ylpentanoic acid) in primary cortical mixed cell cultures exposed to oxidotoxins are greatly improved compared to their parent compounds in the following rank-order: (1) PBN, (2) Trolox, and (3) lipoic acid. In contrast, the protective activity of indole-3-propionic acid was slightly decreased by grafting it on the amphiphilic carrier. Similar observations were made in in vivo experiments using aquatic invertebrate microorganisms, called rotifers, which were exposed to lethal concentrations of nonselective (H2O2) and mitochondria-selective (doxorubicin) oxidotoxins. The conclusion of these studies is that fluorinated amphiphilic PBN, Trolox, and lipoic acid derivatives exhibit very potent protective activities in in vitro and in vivo experiments. The findings demonstrated herein therefore strongly suggest that the amphiphilic character enhances the bioavailability of the antioxidants and allows for a selective targeting of mitochondria.

Original languageEnglish (US)
Pages (from-to)2812-2820
Number of pages9
JournalJournal of Medicinal Chemistry
Volume49
Issue number9
DOIs
StatePublished - May 4 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Ortial, S., Durand, G., Poeggeler, B., Polidori, A., Pappolla, M., Böker, J., Hardeland, R., & Pucci, B. (2006). Fluorinated amphiphilic amino acid derivatives as antioxidant carriers: A new class of protective agents. Journal of Medicinal Chemistry, 49(9), 2812-2820. https://doi.org/10.1021/jm060027e