Fluorometric measurement of tin-protoporphyrin in biological samples

Creuza S. Simionatto; Karl E. Anderson; Shigeru Sassa; George S. Drummond; Attallah Kappas

doi:10.1016/0003-2697(84)90448-2

Fluorometric measurement of tin-protoporphyrin in biological samples

Creuza S. Simionatto, Karl E. Anderson, Shigeru Sassa, George S. Drummond, Attallah Kappas

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.

Original language	English (US)
Pages (from-to)	213-219
Number of pages	7
Journal	Analytical Biochemistry
Volume	141
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90448-2
State	Published - Aug 15 1984
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(84)90448-2

Cite this

@article{0453f2bf86194677b6bdc3305f1c2a3f,

title = "Fluorometric measurement of tin-protoporphyrin in biological samples",

abstract = "Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.",

author = "Simionatto, {Creuza S.} and Anderson, {Karl E.} and Shigeru Sassa and Drummond, {George S.} and Attallah Kappas",

note = "Funding Information: This study was supported by ES01055 Dyson Foundation and the Ogden Corporation.",

year = "1984",

month = aug,

day = "15",

doi = "10.1016/0003-2697(84)90448-2",

language = "English (US)",

volume = "141",

pages = "213--219",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Fluorometric measurement of tin-protoporphyrin in biological samples

AU - Simionatto, Creuza S.

AU - Anderson, Karl E.

AU - Sassa, Shigeru

AU - Drummond, George S.

AU - Kappas, Attallah

N1 - Funding Information: This study was supported by ES01055 Dyson Foundation and the Ogden Corporation.

PY - 1984/8/15

Y1 - 1984/8/15

N2 - Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.

AB - Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.

UR - http://www.scopus.com/inward/record.url?scp=0021163824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021163824&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(84)90448-2

DO - 10.1016/0003-2697(84)90448-2

M3 - Article

C2 - 6496929

AN - SCOPUS:0021163824

SN - 0003-2697

VL - 141

SP - 213

EP - 219

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Fluorometric measurement of tin-protoporphyrin in biological samples

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this