Fluorometric measurement of tin-protoporphyrin in biological samples

Creuza S. Simionatto, Karl E. Anderson, Shigeru Sassa, George S. Drummond, Attallah Kappas

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalAnalytical Biochemistry
Volume141
Issue number1
DOIs
StatePublished - Aug 15 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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