Foamy cells with oligodendroglial phenotype in childhood ataxia with diffuse central nervous system hypomyelination syndrome

K. Wong, Regina C. Armstrong, Kymberly A. Gyure, Alan L. Morrison, Diana Rodriguez, Reuben Matalon, Anne B. Johnson, Robert Wollmann, Enid Gilbert, Tuan Q. Le, Courtney A. Bradley, Kevin Crutchfield, Raphael Schiffmann

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Childhood ataxia with diffuse central nervous system hypomyelination syndrome (CACH) is a recently described leukodystrophy of unknown etiology. To characterize the neuropathological features and gain insight as to the pathogenesis of this disorder, we studied cerebral tissue from six patients with the CACH syndrome. Evaluation of toluidine blue-stained, semithin sections of white matter from CACH patients disclosed unusual cells with ″foamy″ cytoplasm, small round nuclei and fine chromatin. Electron microscopy (EM) revealed cells in the white matter with abundant cytoplasm containing many mitochondria and loosely clustered, membranous structures, but lacking the lysosomal structures seen in macrophages. Further analysis of tissue sections with antibodies and special stains demonstrated that the abnormal cells with abundant cytoplasm labeled with oligodendroglial markers, but did not react with macrophage or astrocytic markers. Double immunolabeling with macrophage and oligodendroglial markers clearly distinguished macrophages from the ″foamy″ oligodendroglial cells (FODCs). Proteolipid protein (PLP) mRNA in situ hybridization demonstrated PLP mRNA transcripts in a high proportion of oligodendrocytes in CACH patients compared to control patients, and PLP mRNA transcript signal in cells, morphologically consistent with FODCs. Normal and pathological brain control tissues did not contain FODCs. These neuropathological findings will be useful pathological identifiers of CACH, and may provide clues to the pathogenesis of this disorder.

Original languageEnglish (US)
Pages (from-to)635-646
Number of pages12
JournalActa Neuropathologica
Volume100
Issue number6
DOIs
StatePublished - 2000

Fingerprint

Leukoencephalopathies
Phenotype
Proteolipids
Macrophages
Cytoplasm
Messenger RNA
Tolonium Chloride
Proteins
Oligodendroglia
Chromatin
In Situ Hybridization
Electron Microscopy
Mitochondria
Coloring Agents
Antibodies
Brain

Keywords

  • Childhood ataxia with diffuse central nervous system hypomyelination (CACH syndrome)
  • Leukodystrophy
  • Myelin
  • Oligodendroglia

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Foamy cells with oligodendroglial phenotype in childhood ataxia with diffuse central nervous system hypomyelination syndrome. / Wong, K.; Armstrong, Regina C.; Gyure, Kymberly A.; Morrison, Alan L.; Rodriguez, Diana; Matalon, Reuben; Johnson, Anne B.; Wollmann, Robert; Gilbert, Enid; Le, Tuan Q.; Bradley, Courtney A.; Crutchfield, Kevin; Schiffmann, Raphael.

In: Acta Neuropathologica, Vol. 100, No. 6, 2000, p. 635-646.

Research output: Contribution to journalArticle

Wong, K, Armstrong, RC, Gyure, KA, Morrison, AL, Rodriguez, D, Matalon, R, Johnson, AB, Wollmann, R, Gilbert, E, Le, TQ, Bradley, CA, Crutchfield, K & Schiffmann, R 2000, 'Foamy cells with oligodendroglial phenotype in childhood ataxia with diffuse central nervous system hypomyelination syndrome', Acta Neuropathologica, vol. 100, no. 6, pp. 635-646. https://doi.org/10.1007/s004010000234
Wong, K. ; Armstrong, Regina C. ; Gyure, Kymberly A. ; Morrison, Alan L. ; Rodriguez, Diana ; Matalon, Reuben ; Johnson, Anne B. ; Wollmann, Robert ; Gilbert, Enid ; Le, Tuan Q. ; Bradley, Courtney A. ; Crutchfield, Kevin ; Schiffmann, Raphael. / Foamy cells with oligodendroglial phenotype in childhood ataxia with diffuse central nervous system hypomyelination syndrome. In: Acta Neuropathologica. 2000 ; Vol. 100, No. 6. pp. 635-646.
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